中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 猪脑心肌炎病毒VP1蛋白单克隆抗体的制备及鉴定 中国兽医科学 2007,37 (1) 24-28
Position: Home > Articles > Preparation and identification of monoclonal antibodies against VP1 protein of porcine encephalomyocarditis virus Chinese Veterinary Science 2007,37 (1) 24-28

猪脑心肌炎病毒VP1蛋白单克隆抗体的制备及鉴定

作  者：
董昕欣;郭鑫;杨汉春;盖新娜;陈艳红;查振林
单  位：
中国农业大学农业部预防兽医学重点开放实验室
关键词：
猪脑心肌炎病毒;VP1蛋白;单克隆抗体
摘  要：
利用纯化的猪脑心肌炎病毒(EMCV)VP1重组蛋白免疫BALB/c小鼠,采用杂交瘤细胞技术,获得了2株稳定分泌抗EMCV VP1蛋白单克隆抗体的杂交瘤细胞株,分别命名为10B和11D。经ELISA测定,2株杂交瘤细胞培养上清效价分别为1∶1 600和1∶800,接种小鼠的腹水效价分别为1∶2.56×106和1∶1.28×106。2株单抗的亚类均为IgG2a,均能特异性识别重组VP1蛋白,但它们识别VP1蛋白的不同表位。间接免疫荧光和免疫组织化学试验证实,2株单抗具有良好的特异性,均能够识别EMCV。
译  名：
Preparation and identification of monoclonal antibodies against VP1 protein of porcine encephalomyocarditis virus
作  者：
DONG Xin-xin,GUO Xin,YANG Han-chun,GE Xin-na,CHEN Yan-hong,ZHA Zhen-lin(Key Laboratory of Preventive Veterinary the Medicine of Ministry of the Agriculture/China Agricultural University,Beijing 100094,China)
关键词：
porcine encephalomyocarditis virus;VP1 protein;monoclonal antibody
摘  要：
Five 8-weeks-old BALB/c mice were immunized with purified recombinant fusion protein VP1 of porcine encephalomyocarditis virus(EMCV).Two hybridoma cell lines designated 10B and 11D steadily secreting monoclonal antibody(McAb) against VP1 protein of EMCV were obtained by hybridoma technology.The titers of the two McAbs in the supernatant culture supernatant were 1∶1600 and(1∶800,) respectively,and the titers in the ascites of the mice were 1∶2.56×10~(6)and 1∶1.28×10~(6),(respectively,) detected by ELISA.The two McAbs belonged to IgG2a subclass and recognized different epitopes of VP1.The McAbs were able to recognize the recombinant VP1 protein of EMCV by Western-blotting,and could react with EMCV by indirect immunofluorescence assay(IFA) and immunohistoche-(mistry,) indicating that the two McAbs exhibit good specificity.

相似文章

导出引用
计量
文章访问数: 1
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46