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Position: Home > Articles > Induction of callus from P. cuspidatum leaves and determination of resveratrol. Medicinal Plant 2011,2 (11)

Induction of callus from P. cuspidatum leaves and determination of resveratrol.

作者：

Lin Ruo-zho

单位：

College of Life Sciences, Shaoxing University, Shaoxing, Zhejiang 312000, Chin

关键词：

leaves;cuspidatum;callus;resveratrol;induced;conten

摘要：

[Objective] To induce the callus from P.cuspidatum leaves and to determine the content of resveratrol.[Method] P.cuspidatum leaves as tissue culture materials were induced on MS induction medium containing 0.2 mg/L 2,4-dichlorophenoxyacetic acid(2,4-D) and 1 mg/L 6-amino-benzyl adenine(6-BA) at 28 ℃ and dark.15 d later,the induced callus was transferred to the same culture medium for 15 d,which were transferred 3 times.Subsequently,the callus was cut and then cultured in the same medium for three times.The content of resveratrol in the callus induced from P.cuspidatum leaves was determined by HPLC.[Result] The callus induced from P.cuspidatum leaves grew well,and its resveratrol content was determined at 0.24 mg/g.[Conclusion] The HPLC method was simple.Convenient,quick and accurate to determine the content of resveratrol in P.cuspidatum leaves.

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Induction of callus from P. cuspidatum leaves and determination of resveratrol.

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