译 名:
Modified Method for the Determination of Vitamin B_1 and Vitamin B_2 in Agricultural Products:
作 者:
Xu Qiaodi, Shen Donglian & Zhang Qi( Central Laboratary, Zhejiang Academy of Agricultural Sciences)
关键词:
Vitamin B_1; Vitamin B_2; Fluorescence; Radial-Pak C_(18)column; Three-standard-addition method
摘 要:
A method of fl0w fluorescent is described to determine Vitamin B_1 with 365nm of exposure and 455nm of emanation.The three-standard-addition method was used meanwhile to remove the interferences caused by pigments and other foreign substances. Vitamin B_2 was pre-purified using Sep-Pak silica gel cartridge and then by Radial-Pak C_(18) column chromatography which was determined by fluorescence detection. The active wave was 365nm with 530nm of emanation.