中国农业期刊集成服务平台

当前位置: 首页 > 文章 > 锈色粒肩天牛幼虫的COⅠ、COⅡ、Cytb和28S基因片段序列分析浙江农林大学学报 2020,37 (2) 303-310

Position: Home > Articles > Sequence analysis of COⅠ, COⅡ, Cytb and 28S gene fragments from Apriona swainsoni larvae Journal of Zhejiang A&F University 2020,37 (2) 303-310

锈色粒肩天牛幼虫的COⅠ、COⅡ、Cytb和28S基因片段序列分析

作者：

叶碧欢;李海波;叶卫邦;陈友吾

单位：

浙江省林业科学研究院

关键词：

森林保护学;锈色粒肩天牛幼虫(斗米虫);COⅠ;COⅡ;Cytb;28S;序列分析;物种鉴别

摘要：

【目的】基于分子生物学手段,探讨可快速准确鉴别锈色粒肩天牛Apriona swainsoni幼虫与其他天牛幼虫的目标基因,为该保健昆虫的食药用安全提供保障。【方法】利用线粒体COⅠ、 COⅡ、 Cytb基因和28S细胞核基因的通用引物分别克隆获得4种基因片段,并进行同源序列检索、多重比对、序列信息分析及进化树构建。【结果】基因克隆最终获得锈色粒肩天牛4种基因序列的片段大小分别为817、 545、 434和1 088 bp。特殊位点分布结果显示:28S的保守位点占比最高,其后依次为Cytb、 COⅠ和COⅡ,变异率则正好相反。COⅠ、 COⅡ和Cytb基因片段的碱基组成和替换信息特点均表现为A+T含量>G+C含量,颠换高于转换,28S基因片段则表现为A+T含量

译名：

Sequence analysis of COⅠ, COⅡ, Cytb and 28S gene fragments from Apriona swainsoni larvae

作者：

YE Bihuan;LI Haibo;YE Weibang;CHEN Youwu;Zhejiang Academy of Forestry;Jinhua Forest Treasure Technology Development Company;

关键词：

forest protection;;Apriona swainsoni larvae;;COⅠ;;COⅡ;;Cytb;;28S;;sequence analysis;;species identification

摘要：

[Objective] Based on molecular bio logical methods, this research was intended to explore the the target genes that could identify Apriona swainsoni larvae from other Cerambycidae larvae, so as to provide a guarantee for the food and drug use of A. swainsonias larvae. [Method] Four different gene fragments were cloned using the general primers of COⅠ, COⅡ, Cytb and 28 S genes before the homologous sequence retrieval,multiple comparison, sequence analysis and molecular phylogenetic tree construction were carried out. [Result](1) The fragments of the four gene sequences obtained by gene cloning were 817, 545, 434 and 1 088 bp,respectively.(2) In light of the distribution of special loci, 28 S had the highest proportion of conserved sites,followed by Cytb, COⅠ and COⅡ, while the variability rate was just the opposite. The base composition and replacement of the COⅠ, COⅡ and Cytb gene fragments were all shown as A +T contents > G +C contents,with the rate of translocation higher than that of the conversion. However, when it came to the 28 S gene fragment, it was A+T cotents< G+C cotents, with the rate of translocation lower than that of the conversion.(3) There existed differences in the sequence structure between mitochondrial genes and nuclear genes with the latter more conservative than the former.(4)The molecular phylogenetic relationship based on those four gene sequences could distinguish A. swainsoni larvae from other Cerambyeidae larvae, such as A. germari.[Conclusion]COⅠ, COⅡ, Cytb and 28 S genes could be used as the molecular reference for the identification of A. swainsoni larvae. [Ch, 1 fig. 4 tab. 42 ref.]

相似文章

用草甘膦除草 [彭菊陔] 中国林业 1989,000 (1) 41
祁连山北坡山杨林病虫害调查及防治策略 [李柏春, 袁虹, 汪有奎, 蒋志成, 杨全生] 植物保护 2006,32 (2) 78-83
水杉原生群落病虫害及其防治 [王柏泉, 艾训儒, 彭诚, 易永梅, 范深厚, 张能望] 植物保护 2003,29 (2) 42-43
南京林业大学7个重点学科列入新一轮国家重点学科 [] 南京林业大学学报(自然科学版) 2007,031 (6) 38
原《林业科学》副主编肖刚柔先生逝世 [] 林业科学 2005 (5) 210
森林病虫害远程诊断及实现 [谭三清, 尹芳志, 张贵, 曹咏梅] 中南林业科技大学学报 2009,29 (6) 189-192
从林业科技论文看森林保护学的发展 [王常禄, 赵世华] 林业科技通讯 1993 (12) 1-3+22
噻虫啉防治花椒蚜虫和红黄蜘蛛药效试验 [马建新, 林源, 郭建] 四川林业科技 2012,33 (2) 92-93
1个含有SDR结构域PKS/NRPS基因的克隆 [原晓龙, 李娟, 李云琴, 王毅] 浙江农林大学学报 2019 (6) 1247-1253
森林保护学实验教学中若干问题浅析与对策 [朱天辉, 李芳莲] 四川农业大学学报 2004,22 (1) 83-85

导出引用

计量

文章访问数: 10

HTML全文浏览量: 0

PDF下载量: 0

锈色粒肩天牛幼虫的COⅠ、COⅡ、Cytb和28S基因片段序列分析

相似文章

所属期刊

浙江农林大学学报

推荐期刊

浙江农林大学学报

中南林业科技大学学报