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Position: Home > Articles > Identification and Phylogenetic Analysis of a Yeast Strain from Biogas Slurry China Biogas 2008,26 (04) 7-10

沼气发酵液中一株酵母菌菌株的鉴定与系统发育分析

作  者:
游银伟;迟晓峰;岳寿松;张昌爱;王梅;王艳芹;刘英
单  位:
山东省农业科学院高新技术研究中心;山东省农业科学院土壤肥料研究所
关键词:
沼气发酵液;季也蒙毕赤酵母(Pichia guilliermondii);5.8S-ITS rDNA;系统发育分析
摘  要:
从沼气发酵液中分离到一株酵母菌,根据形态学特征、生理生化特征和分子生物学方法对其进行了鉴定。克隆了该菌的18S rDNA序列(GenBank接受号:EU784644)和5.8S-ITS rDNA序列(GenBank接受号:EU784645)并进行测序,结果表明18S rDNA全长1739bp,5.8S-ITS rDNA全长569bp。以5.8S-ITS rDNA序列同源性为基础构建了相关属种的系统发育树,结果表明CXF-1与季也蒙毕赤酵母(Pichia guilliermondii)处于同一进化分支中,相似性达99.6%,因此将该菌鉴定为季也蒙毕赤酵母(Pichia guilliermondii)。
译  名:
Identification and Phylogenetic Analysis of a Yeast Strain from Biogas Slurry
作  者:
YOU Yin-wei1,CHI Xiao-feng1,YUE Shou-song1,ZHANG Chang-ai2,LIU Ying2(1.High-Tech Research Center,Shandong Academy of Agricultural Sciences,Jinan 250100,China;2.Soil and Fertilizer Institute,Shandong Academy of Agricultural Sciences,Jinan 250100,China)
关键词:
biogas slurry;Pichia guilliermondii;5.8S-ITS rDNA;phylogenetic analysis
摘  要:
A yeast strain CXF-1 isolated from biogas slurry was identified by morphological,physiological,biochemical characteristics and molecular technology.The 18S rDNA and 5.8S-ITS rDNA of the strain were amplified and sequenced.The results showed that the complete sequence of the 18S rDNA and 5.8S-ITS rDNA were 1788bp and 607bp,respectively.A phylogenetic tree was constructed by comparing with the published 5.8S-ITS rDNA sequences of relative yeast species.In the phylogenetic tree,the strain CXF-1 was the closest relative to Pichia guilliermondii with more than 99.6% sequence similarity.The strain CXF-1 was identified as Pichia guilliermondii.

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