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Position: Home > Articles > Optimization of AFLP System for Hot Pepper Induced by Space Northern Horticulture 2011 (21) 103-105

空间诱变辣椒AFLP反应体系的优化

作  者:
周春阳;郭亚华;谢立波;王雪;高永利;周宇;黄凤兰;彭木
单  位:
黑龙江省农业科学院园艺分院;内蒙古民族大学生命科学学院;东北农业大学园艺学院
关键词:
空间诱变;辣椒;AFLP反应体系;优化
摘  要:
为了建立适合空间诱变辣椒的AFLP反应体系,以3~4叶期的辣椒叶片为试材,对体系中的DNA用量、酶切与连接、预扩增及产物的稀释倍数等关键因素进行了优化,确立了适合空间诱变辣椒的AFLP反应体系。结果表明:采用该体系对空间诱变辣椒进行AFLP分析,能够得到清晰稳定的分子标记图谱。
译  名:
Optimization of AFLP System for Hot Pepper Induced by Space
作  者:
ZHOU Chun-yang1,GUO Ya-hua2,XIE Li-bo2,WANG Xue2,GAO Yong-li2,ZHOU Yu2,HUANG Feng-lan3,PENG Mu3(1.College of Horticulture,Northeast Agricultural University,Harbin,Heilongjiang 150030;2.Horticultural Sub-Academy,Heilongjiang Academy of Agricultural Sciences,Harbin,Heilongjiang 150069;3.College of Life Sciences,Inner Mongolia University for the Nationality,Tongliao,Inner Mongolia 028000)
关键词:
induced by space;hot pepper;AFLP analysis system;optimization
摘  要:
In order to establish a suitable AFLP reaction system of pepper induced by space,taking 3~4 leaf stage of pepper leaves as test materials,several significant influencing factors,including the dosage of template DNA,enzyme digestion and ligation,pre-amplification and the dilute of pre-amplification products were optimized,and a suitable AFLP reaction system for hot pepper induced by space was constructed.The results showed that by this optimized system,a clear and credible AFLP fingerprint could be obtained.

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