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当前位置: 首页 > 文章 > 狗蔷薇类根体和类原球茎发生发育的组织学研究 园艺学报 2014,41 (6) 1167-1174
Position: Home > Articles > Studies on the Histology and Ultrastructure of the Initiation of Rhizoids and Protocorm-like Bodies(PLBs)Induced from the Leaf Explants of Rosa canina Acta Horticulturae Sinica 2014,41 (6) 1167-1174

狗蔷薇类根体和类原球茎发生发育的组织学研究

作  者：
张建甫;田传卫;毕玲;赵梁军
单  位：
中国农业大学观赏园艺与园林系
关键词：
狗蔷薇;叶片外植体;类根体;类原球茎;发生与发育;组织学研究;超微结构
摘  要：
以狗蔷薇叶片为对象观察其类根体和类原球茎发生发育的过程。发现叶片外植体叶柄维管束的原形成层细胞在2,4-D的诱导下分裂并扩大形成狭长的微管组织细胞,进一步衍生形成根源基细胞,根源基细胞分裂形成根尖分生组织,并最终形成类根体。类根体具有根冠、生长点、伸长区和根毛区,类根体只有初生结构,不具备次生结构。携带有愈伤组织的类根体在含TDZ的1/2MS培养基和光照协调作用下,根尖分生组织消失并分化成成熟的薄壁细胞,中柱鞘上部的细胞形成胚性愈伤组织,其分化为胚性细胞并形成新的分生中心,突破皮层和表皮,进而形成类原球茎。
译  名：
Studies on the Histology and Ultrastructure of the Initiation of Rhizoids and Protocorm-like Bodies(PLBs)Induced from the Leaf Explants of Rosa canina
作  者：
ZHANG Jian-fu;TIAN Chuan-wei;BI Ling;ZHAO Liang-jun;Department of Ornamental Horticulture and Landscape Architecture,China Agricultural University;Department of Urban Construction,Beijing City University;
关键词：
Rosa canina;;leaf explant;;rhizoid;;protocorm-like bodies;;initiation and development;;histology;;ultrastructure
摘  要：
Leaf explants of Rosa canina were used to investigate the origins of the rhizoids and protocorm-like bodies formation. Histological studies revealed that remained procambium cells in the vascular bundles were induced by 2,4-D,then divided and enlarged to form long but narrow vascular tissue cells that functioned as pluripotent stem cells with a tendency to form root primordia which further developed to form root meristems. The rhizoids have the same anatomical structure with the roots growing in vitro,including root cap,root meristematic zone,elongation zone and maturation zone,and the rhizoids only has the primary structure not secondary structure. Incubating the rhizoids carrying calli under light/dark conditions on 1/2MS medium containing TDZ,the tips of rhizoids could develop into protocorm-like bodies(PLBs). Histological and ultrastructure observation revealed that the cells in the tip of rhizoids firstly differentiated into large parenchyma cells,these cells adjacent to the epidermis dedifferentiated to form a great many proembryo calli under the conditions of light and TDZ. And then the embryogenic cells divided and enlarged to form PLBs.

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