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当前位置: 首页 > 文章 > 杉木ClWRKY44基因克隆及其表达特性分析 南京林业大学学报(自然科学版) 2022 (1) 203-209
Position: Home > Articles > Cloning and expression characteristics of ClWRKY44 gene from Cunninghamia lanceolata Journal of Nanjing Forestry University(Natural Sciences Edition) 2022 (1) 203-209

杉木ClWRKY44基因克隆及其表达特性分析

作  者：
林莉莉;胡安琪;陈钢;张霁月;曹光球;曹世江
单  位：
国家林业和草原局杉木工程技术研究中心;福建农林大学林学院;华川技术有限公司
关键词：
杉木;基因克隆;基因表达分析;蛋白亚细胞定位;逆境胁迫
摘  要：
【目的】以杉木幼叶为试验材料,通过克隆杉木ClWRKY44基因,分析其表达特性,为揭示杉木抗逆生理的分子机制提供理论依据。【方法】采用RT-PCR技术克隆杉木ClWRKY44基因,对其进行生物信息学分析和亚细胞定位研究,并利用实时荧光定量PCR技术分析其表达情况。【结果】杉木ClWRKY44基因的开放阅读框(ORF)为1 776 bp,编码591个氨基酸,具有WRKY结构域。系统进化树分析结果显示ClWRKY44基因与拟南芥(Arabidopsis thaliana)ATWRKY44的亲缘关系较密切,其在杉木的不同器官(叶、根、茎)中均有表达,嫩叶中的表达量最高,茎中次之,根中最低。低温胁迫处理6 h,ClWRKY44基因相对表达量最高,约是对照组的79.8倍。干旱胁迫处理24 h,ClWRKY44基因在高浓度干旱胁迫处理下的相对表达量最高,约是对照组的46.6倍。【结论】杉木ClWRKY44基因编码的蛋白质序列与拟南芥ATWRKY44的同源性很高,在调控植物逆境胁迫应答过程中可以发挥功能,为杉木幼苗生长发育的适应性提供参考。
译  名：
Cloning and expression characteristics of ClWRKY44 gene from Cunninghamia lanceolata
作  者：
LIN Lili;HU Anqi;CHEN Gang;ZHANG Jiyue;CAO Guangqiu;CAO Shijiang;College of Forestry, Fujian Agriculture and Forestry University;Huachuan Technology Company Limited;Chinese Fir Engineering Technology Research Center of the National Forestry and Grassland Administration;
关键词：
Cunninghamia lanceolate;;gene cloning;;gene expression analysis;;protein subcellular localization;;stresses
摘  要：
【Objective】The aim of this study is to clone the Cunninghamia lanceolata ClWRKY44 gene and analyze its expression characteristics, so as to provide a theoretical basis for revealing the molecular mechanism of stress resistance physiology of Cunninghamia lanceolata. 【Method】 We cloned the Chinese fir ClWRKY44 gene by using RT-PCR technology, conducted bioinformatics analysis and subcellular localization study, and used real-time fluorescent quantitative PCR technology to analyze its expression. 【Result】The open reading frame(ORF)of ClWRKY44 gene is 1 776 bp, which encodes 591 amino acids and has a WRKY domain. Phylogenetic tree analysis showed that ClWRKY44 gene was closely related to Arabidopsis thaliana ATWRKY44. It was expressed in different organs(leaves, roots, stems) of Cunninghamia lanceolata. After 6 h of low temperature stress treatment, the relative expression of ClWRKY44 gene was the highest, about 79.8 times that of the control group. After 24 h of drought stress treatment, the relative expression of ClWRKY44 gene was the highest under high concentration drought stress treatment, about 46.6 times that of the control group. 【Conclusion】The protein sequence encoded by Chinese fir ClWRKY44 gene has high homology with Arabidopsis ATWRKY44. It can play a role in regulating plant stress response, and provide a reference for the adaptability of Chinese fir seedling growth and development.

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