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当前位置: 首页 > 文章 > 保育和育肥猪舍气溶胶微生物组成和四环素类抗性基因丰度研究 中国畜牧杂志 2019 (5) 99-102,106
Position: Home > Articles > 保育和育肥猪舍气溶胶微生物组成和四环素类抗性基因丰度研究 Chinese Journal of Animal Science 2019 (5) 99-102,106

保育和育肥猪舍气溶胶微生物组成和四环素类抗性基因丰度研究

作  者：
许铭洙;杨勇;郭振东;严鸿林;张宏福;刘静波
单  位：
西南科技大学生命科学与工程学院
关键词：
保育猪舍;育肥猪舍;气溶胶;微生物组成;四环素类抗性基因
摘  要：
本研究旨在比较保育舍和育肥舍气溶胶微生物组成和四环素类抗性基因丰度的差异.采集8个保育舍和8个育肥舍的空气样品,用RT-qPCR方法检测特定微生物类群的相对丰度和四环素类抗性基因的绝对丰度.结果表明:与保育舍相比,育肥舍气溶胶微生物组中厚壁菌门、乳酸杆菌属、罗伊氏乳杆菌和约氏乳杆菌丰度显著提高(P<0.05),而拟杆菌门、梭菌属和普雷沃氏菌属丰度显著降低(P<0.05);育肥舍气溶胶中TetB、TetH、TetZ、TetO、TetQ和TetW的拷贝数显著低于保育舍(P<0.05);主成分分析表明,保育舍和育肥舍气溶胶可按照微生物丰度和四环素类抗性基因丰度进行聚类.由此可见,不同猪舍类型微生物组成存在明显差异,保育舍抗性基因丰度显著高于育肥舍.
作  者：
XU Ming-zhu;YANG Yong;GUO Zhen-dong;YAN Hong-lin;ZHANG Hong-fu;LIU Jing-bo;School of Life Science and Engineering, Southwest University of Science and Technology;Institute of Military Veterinary, Academy of Military Medical Sciences;State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences;
单  位：
XU Ming-zhu%YANG Yong%GUO Zhen-dong%YAN Hong-lin%ZHANG Hong-fu%LIU Jing-bo%School of Life Science and Engineering, Southwest University of Science and Technology%Institute of Military Veterinary, Academy of Military Medical Sciences%State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences
关键词：
Nursery buildings;;Finishing buildings;;Bioaerosol;;Microbial composition;;Antibiotic resistance genes
摘  要：
The present study was conducted to compare the microbial composition and abundances of tetracycline resistance genes in bioaerosols between nursery buildings and finishing buildings. Airborne samples from eight nursery buildings and eight finishing buildings were collected to determine the abundances of specific microbes and the copies of antibiotic resistance genes by using RT-qPCR. The results showed that the abundances of phylum Firmicutes, Lactobacillus,Lactobacillus reuteri and Lactobacillus johnsonii were higher in finishing buildings than those in nursery buildings(P<0.05). In addition,the abundances of phylum Bacteroidetes, Clostridia and Prevotella were lower in finishing buildings than those in nursery buildings(P<0.05). The primary component analyses showed that the bioaerosols of swine confinement buildings could be clustered by bacterial abundances or copy numbers of antibiotic resistance genes. In conclusion, the bioaerosol microbial composition in nursery buildings was different from that in finishing buildings, and the copies of antibiotic resistance genes were higher in nursery buildings than those in finishing buildings.

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