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当前位置: 首页 > 文章 > 气相色谱法测定蝉花中角鲨烯的含量 安徽农业科学 2016,44 (28) 94-95+98
Position: Home > Articles > Determination of Squalene in Cordyceps cicadae Shing by Gas Chromatography Journal of Anhui Agricultural Sciences 2016,44 (28) 94-95+98

气相色谱法测定蝉花中角鲨烯的含量

作  者：
凡军民;贾君;谢春芹;韩艳丽
单  位：
江苏农林职业技术学院
关键词：
蝉花;角鲨烯;气相色谱法
摘  要：
[目的]建立毛细管气相色谱(GC)测定蝉花中角鲨烯含量的方法。[方法]采用气相色谱法测定蝉花中角鲨烯,并对不同部位天然蝉花的角鲨烯含量进行比较,确定蝉花中角鲨烯含量测定的最佳条件。[结果]1 g蝉花经超声波提取后,经1 m L 0.5 mol/L的氢氧化钠-甲醇溶液40℃皂化5 min,再经正已烷萃取净化,采用GC法测定角鲨烯含量。该方法在角鲨烯质量浓度为21.43~214.30μg/m L(r=0.999 2)范围内线性关系良好,方法精密度良好(RSD=3.71%),加标回收率为77.80%~97.32%,平均加标回收率(n=9)为88.10%。[结论]气相色谱法简单快速,污染小,检测灵敏度高,可用于蝉花中角鲨烯含量的测定。
译  名：
Determination of Squalene in Cordyceps cicadae Shing by Gas Chromatography
作  者：
FAN Jun-min;JIA Jun;XIE Chun-qin;Jiangsu Vocational College of Agriculture and Forestry;
关键词：
Cordyceps cicadae Shing;;Squalene;;Gas chromatography
摘  要：
[Objective] To establish a method of capillary gas chromatography to detect the content of squalene in Cordyceps cicadae.[Method]Squalene in Cordyceps cicadae Shing was detected by gas chromatography. And the squalene contents in different parts of C. cicadae were compared. The optimal condition for the detection of squalene in C. cicadae was obtained.[Result] C. cicadae( 1 g) was extracted by ultrasonic extraction,the product was methyl esterified at 40 ℃ for 5 min by 1 m L of 0. 5 mol/L sodium hydroxide-methanol solution and then extracted by nhexane. The squalene could be determined quantitatively by GC. When the squalene concentration was from 21. 43 to 214. 30 μg/m L,there was a good linear relation( r = 0. 999 2) and a perfect method precision( RSD = 3. 71%),and the recovery rate was 77. 80%-97. 32%,and the average recovery rate was 88. 10%( n = 9).[Conclusion]This method was simple and rapid with low pollution and high sensitivity,and it is suitable for determination of squalene content in C. cicadae.

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