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金黄色葡萄球菌标准物质的研制

作  者:
薛蕾;隋志伟;张玲;王晶;傅博强;刘瑛颖;郝林
单  位:
山西农业大学食品科学与工程学院;中国计量科学研究院
关键词:
金黄色葡萄球菌;冷冻干燥;保护剂;标准物质
摘  要:
将金黄色葡萄球菌添加到复合保护剂中,经冻干制备成干粉状标准物质,通过比较冻干前后活菌数来验证保护剂保护效果,并研究标准物质的均匀性和稳定性。结果表明复合保护剂的保护率达到51.74%,可以在冻干过程中有效保护菌体,制备的金黄色葡萄球菌标准物质均匀性良好,可以稳定保存半年。该标准物质可用于食品检测实验室质量控制和食品中金黄色葡萄球菌检测结果的评价。
译  名:
Preparation of Staphylococcus aureus Reference Material
作  者:
XUE Lei;SUI Zhiwei;ZHANG Ling;WANG Jing;FU Boqiang;LIU Yingying;HAO Lin;National Institute of Metrology;College of Food Science and Engineering, Shanxi Agricultural University;
关键词:
Staphylococcus aureus;;freeze-drying;;protectant;;reference material
摘  要:
A reference material of Staphylococcus aureus was prepared by freeze-drying Staphylococcus aureus cells in the presence of a composite cryoprotectant. The efficacy of the cryoprotectant was verified by comparing the numbers of viable bacterial cells before and after the freeze-drying, and the homogeneity and stability of the standard substance were studied. The results showed that the composite protective agent could protect 51.74% of the viable bacterial cells. The homogeneity and stability after six months of storage of the reference material of Staphylococcus aureus were good. The reference material can be used for quality control in food testing laboratories and evaluation of food test results.

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