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Position: Home > Articles > Biochemical Characteristics of Phenoloxidase from Portunus trituberculatus FOOD SCIENCE 2010,31 (1) 172-176

三疣梭子蟹酚氧化酶的生化特性研究

作  者:
郑丹;段青源;朱励华;钟惠英;杨家锋;汪杰
单  位:
宁波市海洋与渔业研究院;宁波市渔业环境与产品质量检验监测中心
关键词:
三疣梭子蟹;酚氧化酶;褐变
摘  要:
对三疣梭子蟹酚氧化酶的生化特性进行研究。结果表明:以邻苯二酚为底物,酚氧化酶在pH7.8~8.4时最稳定,最适pH值为8.2。温度55℃时,酚氧化酶活力最大,在25~40℃时,热稳定性最好。酶促褐变反应动力学符合米氏方程,温度为30℃,pH7.2时,米氏常数Km=0.531mol/L,最大反应速率0.049A530nm/min。选用的12种抑制剂对三疣梭子蟹酚氧化酶均有一定的抑制效果,以植酸抑制效果最强,焦亚硫酸钠、L-半胱氨酸、草酸次之,抗坏血酸效果较好。
译  名:
Biochemical Characteristics of Phenoloxidase from Portunus trituberculatus
作  者:
ZHENG Dan1,DUAN Qing-yuan2,,ZHU Li-hua1,ZHONG Hui-ying1,YANG Jia-feng1,WANG Jie1(1.Ningbo Inspection and Monitor Centre for Product Quality and Fishery Environment,Ningbo 315012,China;2.Ningbo Academy of Ocean and Fishery,Ningbo 315012,China)
关键词:
Portunus trituberculatus;phenoloxidase;melanin
摘  要:
Crude phenoloxidase(PO) was extracted from Portunus trituberculatus and characterized biochemically.This enzyme exhibited an optimal pH of 8.2 and temperature of 55 ℃ for catalyzing the oxidation of pyrocatechol as a substrate.Optimal enzyme stability was observed in the ranges of pH 7.8-8.4 and 25-40 ℃.Browning reaction catalyzed by this enzyme obeyed the Michaelis-Menten equation.The values of Km and Vmax at 30 ℃ and pH 7.2 were calculated to be 0.531 mol/L and 0.049 A530nm/min,respectively.Twelve inhibitors tested all had a certain inhibitory effect against this enzyme,among which phytic acid was the strongest inhibitor,followed in turn by sodium metabisulfite,L-cysteine,oxalic acid and ascorbic acid.

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