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Position: Home > Articles > Studies on High Quality Somatic Embryogenesis Applied to Artificial Seed of Euphorbia pulcherrima Willd. Acta Horticulturae Sinica 2006,33 (1) 175-178

适于制作人工种子的一品红体细胞胚的培养

作  者:
肖颖;王刚
单  位:
河北师范大学生命科学学院
关键词:
一品红;愈伤组织;体细胞胚发生;人工种子
摘  要:
MS+2,4-D 2.0 mg.L-1+6-BA 0.5 mg.L-1固体培养基较适合一品红苞片诱导愈伤组织。MS+2,4-D 1.0 mg.L-1+6-BA 0.5 mg.L-1+水解酪蛋白80 mg.L-1固体培养基上体细胞胚分化率最高。活性炭、MS培养基和悬浮振荡培养有利于高频率体细胞胚发生。球形、心形及梨形胚及时转入添加NAA 0.2mg.L-1、6-BA 0.5 mg.L-1及水解酪蛋白80 mg.L-1的MS液体培养基中,有利于高质量体细胞胚发生。子叶期胚及时转入添加ABA 0.5 mg.L-1的上述培养基中,会提高正常体细胞胚的百分率。用高质量(子叶期)的一品红体细胞胚包埋制作人工种子,在无菌条件下萌发率为58.1%。
译  名:
Studies on High Quality Somatic Embryogenesis Applied to Artificial Seed of Euphorbia pulcherrima Willd.
作  者:
Xiao Ying~(1,2) and Wang Gang~1(()~1The College of Life Science,Hebei Normal University,Shijiazhuang 050016,China;()~2Department of Chemistry,Shijiazhuang College,Shijiazhuang 050801,China)
关键词:
Euphorbia pulcherrima;Callus;Somatic embryogenesis;Artificial seed
摘  要:
MS agar medium supplemented with 24-D 2.0 mg·L~(-1) and 6-BA 0.5 mg·L~(-1)was proved to be the best for inducing callus from the leaves of Euphorbia pulcherrima Willd.It was suitable for somatic embryogenesis when the concentration of 24-D was reduced to 1.0 mg·L~(-1).Activated carbon,MS medium and suspension culture were suitable for high frequency somatic embryogenesis.If globular embryoids,heart-shaped embryoids and pear 6-BA shaped embryoids were transferred into MS medium supplemented with NAA 0.2 mg·L~(-1),6-BA 0.5 mg·L~(-1) and casein hydrolyzed 80 mg·L~(-1),the quality of somatic embryos would be improved.If cotyleden embryoids were transferred in time into a forementioned medium added on ABA 0.5(mg·)L~(-1),the rate of normal somatic embryogenesis would be increased.

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