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Position: Home > Articles > Analysis of Lactofen Enantiomers by HPLC Agrochemicals 2008,47 (7) 43-44

高效液相色谱法分析乳氟禾草灵对映体

作  者:
阎峰;关瑾;崔畅;丛璐;刘晓霞
单  位:
东北大学理学院;沈阳化工学院
关键词:
高效液相色谱法;乳氟禾草灵;对映体;含量测定
摘  要:
采用手性固定相高效液相色谱法在对映体水平上建立了乳氟禾草灵的含量分析方法。采用Chiralpak AS-H手性柱,以正己烷-异丙醇(体积比为90∶10)为流动相,流速为0.6mL/min,检测波长281nm。两对映体质量浓度在2.510~62.75mg/L范围内,线性关系良好(r=0.9991)。保留时间的相对标准偏差分别为0.48%和0.49%(n=6),峰面积的相对标准偏差分别为1.75%和1.85%(n=6)。该方法被验证并应用于合成的S-乳氟禾草灵对映体过量百分率的测定。
译  名:
Analysis of Lactofen Enantiomers by HPLC
作  者:
YAN Feng1,2, GUAN Jin2, CUI Chang2, CONG Lu2, LIU Xiao-xia1 (1.Northeastern University, College of Sciences, Shenyang 110004, China; 2.Shenyang Institute of Chemical Technology, Shenyang 110004, China)
关键词:
HPLC; lactofen; enantiomer; analysis
摘  要:
A method for the analysis of Lactofen was established by HPLC using Chiralpak AS-H chiral station-ary phase. Hexane/isopropanol (90:10 by vol) mobile phase was used with a flow rate of 0.6 mL/min and detection wavelength of 281nm. A good linearity was observed for each enantiomer in the concentration range of 2.510 to 62.75 mg/L with r=0.999 1. The RSDs for the retention time were 0.48 and 0.49%(n=6), respectively. The RSDs for peak area were 1.75 and 1.85%(n=6), respectively. The method was validated and applied to the determina-tion of enantiomeric excess of S-lactofen.

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