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当前位置: 首页 > 文章 > 基于蛋白质组学研究红光对番茄果实糖代谢的影响 园艺学报 2018 (10) 1941-1951
Position: Home > Articles > Studies on the Sugar Metabolism Mechanism of Tomato Fruit Under Red Light Revealed by Proteomic Analysis Acta Horticulturae Sinica 2018 (10) 1941-1951

基于蛋白质组学研究红光对番茄果实糖代谢的影响

作  者：
董飞;王传增;孙秀东;张现征;任煜倩;周丽君;王丹;刘世琦
关键词：
番茄;蛋白组学;红光;糖代谢
摘  要：
以‘Micro-Tom’番茄(Solanumlycopersicum L.)为试材,待种子发芽后30d移入光质实验室,分别用红光和白光(对照)处理植株,在果实绿熟期、转色期、成熟期取样,测定果实可溶性糖含量,进行蛋白质组学分析。结果表明,在果实发育过程中红光处理的番茄果实可溶性糖含量显著高于对照。蛋白组数据显示,与白光对照相比,在绿熟期,红光处理的番茄果实有46个差异蛋白,其中4个与糖代谢有关;在转色期鉴定出134个差异蛋白,其中4个与糖代谢有关;在成熟期鉴定出65个差异蛋白,其中7个与糖代谢有关。在15个与糖代谢有关的差异蛋白中,9个上调表达,6个下调表达。对差异蛋白进行mRNA水平的验证,发现只有甘油醛–3–磷酸脱氢酶(GAPN)、乙酰辅酶A羧化酶(ACACA)在蛋白质和mRNA水平表达不一致,其他蛋白在两个水平表达一致,这说明转录水平并不总是与翻译水平一致。由此可见,红光处理通过影响一些与糖代谢有关的基因和蛋白来正调控番茄果实糖含量。
译  名：
Studies on the Sugar Metabolism Mechanism of Tomato Fruit Under Red Light Revealed by Proteomic Analysis
作  者：
DONG Fei;WANG Chuanzeng;SUN Xiudong;ZHANG Xianzheng;REN Yuqian;ZHOU Lijun;WANG Dan;LIU Shiqi;College of Horticulture Science and Engineering,Shandong Agricultural University,State Key Laboratory of Crop Biology,Ministry of Agriculture Key Laboratory of Biology and Genetic Improvement of Horticultural Crops in Huanghuai Region;Shandong Institute of Pomology;
单  位：
DONG Fei%WANG Chuanzeng%SUN Xiudong%ZHANG Xianzheng%REN Yuqian%ZHOU Lijun%WANG Dan%LIU Shiqi%College of Horticulture Science and Engineering,Shandong Agricultural University,State Key Laboratory of Crop Biology,Ministry of Agriculture Key Laboratory of Biology and Genetic Improvement of Horticultural Crops in Huanghuai Region%Shandong Institute of Pomology
关键词：
tomato;;proteomics;;red light;;sugar metabolism
摘  要：
We used tomato(Solanum lycopersicum L.)‘Micro-Tom'as the test material,after 30 days of seed germination. The seedlings were transferred to the artificial climate chamber with red and white(the control)lights. The fruits were harvested at the mature green,breaker and ripe stages,then we determined the fruit soluble sugar content and proteomic. The results showed that the soluble sugar content under red-treatment was higher than the control during the development of tomato fruit,and the difference was significant when compared with the control. The proteomic data showed that,compared with the control,46,134 and 65 differentially abundant proteins were identified in the red-treated tomato fruit,and four,four and seven of them were related to sugar metabolism in the mature green,breaker and ripe stages,respectively. Among the fifteen differentially abundant proteins related to sugar metabolism,nine were up-regulated and six were down-regulated. The differential abundances of the fifteen proteins were validated by determining the expression levels of the corresponding mRNAs by qRT-PCR. With two exceptions of glyceraldehyde-3-phosphate dehydrogenase( GAPN) and acetyl-CoA carboxylase(ACACA),the remaining genes exhibited a similar expression pattern between their mRNA and protein levels,confirming the differential gene expression levels and revealing that transcription levels are not always concomitant to the translation level. Based on the comprehensive analysis,the red light treatment can regulate the sugar content of tomato fruit by regulating some genes and proteins related to sugar metabolism.

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