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Position: Home > Articles > Insect resistance to Bt-transgenic cotton by seed bioassay Plant Protection 2004,30 (4) 24-28

种子生测法鉴定转Bt基因棉抗虫性

作  者:
王清;李长福;吴振廷;郑厚今;王学林;林华峰
单  位:
安徽农业大学植物保护系;安徽省种子总公司
关键词:
抗病虫育种;种子生测法;转Bt基因棉;抗性鉴定
摘  要:
通过室内应用单粒种子生测法鉴定转Bt基因棉抗虫性,明确了单粒棉种室内生测鉴定转Bt基因棉抗虫性的最佳生测条件。将转Bt基因棉种子25℃萌动72 h后匀浆,与0.45 g饲料混匀,以2日龄棉铃虫幼虫为试虫,测定其死亡率。建立了相应的抗性标准:棉铃虫幼虫校正死亡率小于25%为中抗,处于25%-50%为抗,50%-65%为高抗,大于65%为特高抗。准确性验证表明,种子生测法同叶片生测法的结果相符,具有较好的准确性。
译  名:
Insect resistance to Bt-transgenic cotton by seed bioassay
作  者:
WANG Qing, LI Chang-fu, WU Zhen-ting, ZHENG Hou-jin, WANG Xue-lin, LIN Hua-feng(Department of Plant Protection , Anhui Agricultural University, Hefei 230036, China; General Seeds Company of Anhui Province, Hefei 230036, China)
关键词:
resistance breeding to pests; seed bioassay; Bt-transgenic cotton; identification
摘  要:
The bioassay with neonate larvae of bollworm was carried out to test their resistance to Bt-transgenic cotton using its seed. The results showed that the bioassay with 2nd instar larvae was appropriate by soaking a seed for 72 hours at 25℃, and mixing it with 0.45 g artificial foods. The evaluation criterion was established as follows: 25% or smaller larva mortality was medium resistance, 25%-50% resistance, 50%-65% high resistance, and 65% or above particularly high resistance. It was verified that the bioassay results by using cotton seeds of different insect resistance level agreed with those by using the leaves of Bt-transgenic cotton and this method was stable and reliable.

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