作 者：
赵芳;陈晖;王根;高超;赵国栋;马晨;杨开伦
关键词：
绵羊;胃肠道;内容物;瘤胃保护性5-羟基色氨酸;褪黑素
摘 要：
本试验旨在研究绵羊胃肠道内容物中褪黑素的分布特点,探究瘤胃保护性5-羟基色氨酸对绵羊胃肠道内容物和血浆中5-羟基色氨酸、褪黑素含量的影响,探讨通过5-羟基色氨酸调节绵羊肠道中褪黑素合成的可能性。试验选取健康、平均体重为(47.79±3.70) kg的3岁哈萨克母羊15只,按体重分为3组,每组5只,分别为对照组和试验Ⅰ、Ⅱ组。每天每只羊的粉状精料饲喂量为体重的1.2%,玉米青贮饲喂量为0.9 kg,混合干草自由采食,在此基础上,试验Ⅰ、Ⅱ组羊只分别饲喂111、222 mg/kg BW瘤胃保护性5-羟基色氨酸,进行15 d的饲养试验。结果表明:绵羊胃肠道内容物中5-羟基色氨酸含量的分布特点是盲肠>空肠、结肠>瘤胃液、十二指肠、回肠;褪黑素含量分布特点是十二指肠>瘤胃液、空肠>回肠、盲肠>结肠。除盲肠和结肠外,试验Ⅰ、Ⅱ组绵羊胃肠道内容物中5-羟基色氨酸含量极显著高于对照组(P<0.01)。试验Ⅱ组十二指肠内容物中5-羟色胺、N-乙酰-5-羟色胺含量极显著高于对照组(P<0.01),结肠内容物中5-羟色胺、N-乙酰-5-羟色胺含量显著高于对照组(P<0.05);试验Ⅰ组空肠、回肠内容物中5-羟色胺、N-乙酰-5-羟色胺含量极显著低于对照组、试验Ⅱ组(P <0.01)。试验Ⅰ、Ⅱ组结肠内容物中褪黑素含量极显著高于对照组(P<0.01)。试验Ⅰ、Ⅱ组十二指肠、空肠内容物中5-羟基吲哚乙酸含量极显著低于对照组(P<0.01),但试验Ⅱ组盲肠、结肠内容物中5-羟基吲哚乙酸含量极显著高于对照组(P<0.01)。试验Ⅱ组血浆中5-羟基色氨酸、5-羟色胺、褪黑素含量均显著或极显著高于对照组(P<0.05或P<0.01)。由此可见,绵羊胃肠道内容物中5-羟基色氨酸含量的分布特点是:盲肠>空肠、结肠>瘤胃液、十二指肠、回肠;褪黑素含量分布特点是:十二指肠>瘤胃液、空肠>回肠、盲肠>结肠。瘤胃保护性5-羟基色氨酸补喂量为222 mg/kg BW时能提高胃肠道内容物中5-羟基色氨酸含量,但对5-羟色胺、褪黑素含量的影响在各胃肠段表现不同。瘤胃保护性5-羟基色氨酸补喂量为111、222 mg/kg BW时均能提高绵羊血浆中5-羟基色氨酸、5-羟色胺、褪黑素含量。
译 名：
Effects of Rumen Protected 5-Hydroxytryptophan on Contents of 5-Hydroxytryptophan,Melatonin in Gastrointestinal Tract Digesta and Plasma of Sheep
作 者：
ZHAO Fang;CHEN Hui;WANG Gen;GAO Chao;ZHAO Guodong;MA Chen;YANG Kailun;Xinjiang Agricultural University,Xinjiang Key Laboratory of Meat & Milk Production Herbivore Nutrition;
单 位：
ZHAO Fang%CHEN Hui%WANG Gen%GAO Chao%ZHAO Guodong%MA Chen%YANG Kailun%Xinjiang Agricultural University,Xinjiang Key Laboratory of Meat & Milk Production Herbivore Nutrition
关键词：
sheep;;gastrointestinal tract;;digesta;;rumen protected 5-HTP;;melatonin
摘 要：
This study aimed to investigate the distribution of melatonin in gastrointestinal tract digesta of sheep,to study the effects of rumen protected 5-hydroxytryptophan(5-HTP) on contents of 5-HTP,melatonin in gastrointestinal tract digesta and plasma of sheep,and to explore the possibility of regulating sheep gastrointestinal tract melatonin synthesis by 5-HTP.Fifteen 3-year-old Kazak sheep with an average body weight of(47.79 ±3.70) kg were divided into 3 groups(control group,trial group Ⅰ and trial group Ⅱ) with 3 sheep per group.All sheep were fed 1.2% body weight powder concentrate,and 0.9 kg corn silage per sheep per day,and had free feeding mixed with hay,additionally,each sheep in trail groups Ⅰ and Ⅱ was fed 111 and222 mg/kg BW rumen protected 5-HTP,respectively.The feeding experiment lasted for 15 days.The results showed that the distribution characteristics of the content of 5-HTP in gastrointestinal tract digesta of sheep were:caecum >jejunum,colon>rumen fluid,duodenum,ileum; and the distribution characteristics of melatonin content were:duodenum > rumen fluid,jejunum > ileum,caecum > colon.Expect caecum and colon,the content of 5-HTP in gastrointestinal tract digesta of sheep in trail groups Ⅰ and Ⅱ was significantly higher than that in the control group(P < 0.01).The contents of 5-hydroxytryptamine(5-HT) and N-acetylserotonin(NAS) in duodenum digesta in trail group Ⅱ were significantly higher than those in the control group(P<0.01),and the contents of 5-HT and NAS in colon digesta in trail group Ⅱ were significantly higher than those in the control group(P < 0.05); the contents of 5-HT and NAS in jejunum and ileum digesta in trial group Ⅰ were significantly lower than those in the control group and trial group Ⅱ(P<0.01).The content of melatonin in colon digesta in trail groups Ⅰ and Ⅱ was significantly higher than that in the control group(P<0.01).The content of 5-hydroxyindoleaceticacid(5-HIAA) in duodenum and jejunum digesta in trail groupsⅠ and Ⅱ was significantly lower than that in the control group(P<0.01),however,the content of 5-HIAA in caecum and colon digesta in trial group Ⅱ was significantly higher than that in the control group(P<0.01).The contents of 5-HTP,5-HT and melatonin in plasma in trial group Ⅱ were significantly higher than those in the control group(P<0.05 or P<0.01).Therefore,the distribution characteristics of the 5-HTP content in gastrointestinal tract digesta of sheep are:caecum >jejunum,colon>rumen fluid,duodenum,ileum,and the distribution characteristics of melatonin content are:duodenum >rumen fluid,jejunum >ileum,caecum >colon.Rumen protected 5-HTP supplementation at 222 mg/kg BW can increase the content of 5-HTP in gastrointestinal tract digesta,but its effect on 5-HT and melatonin contents perform differently in different gastrointestinal tract.Rumen protected 5-HTP supplementation at 111 and 222 mg/kg BW can increase the contents of 5-HTP,5-HT and melatonin in plasma of sheep.
