Separation and Identification of Liszt Bacteria in Fresh Milk and Meat from Some Parts of Xinjiang

LIANG Yong-wen,WANG Jing-mei,SHANG Wen-jing,ZHAO Xin-fei,YAN Gen-qiang (College of Animal Science,Shihezi University,Shihezi Xinjiang 832000,China)

fresh meat and milk;;Liszt bacteria;;Separation;;PCR identification

[Objective]To investigate infection and master the ecological distribution in animal products of Listeria monocytogenes in fresh meat and milk from parts of Xinjiang.[Method]A total of 680 samples that were from fresh meat or milk of cattle,sheep,pigs and poultry from Shihezi,Kuitun and Ermin,etc were collected.We used the method of two - step growth bacteria to isolate the Listeria from these samples according to GB,then identified them by using bacteria biochemical identification and PCR method,and tested the virulence by mice infection experiment.[Result]The result showed that eight strains Listeria were isolated from the 680 samples,the carrier rate was 1.14%;there were 4 strains mononuclear Listeria(LM),which the carrier rate was 0.6%;and they would lead the incidence of death in mice.These revealed the low carrier rate of Listeria in fresh meat and milk in some areas in Xinjiang,but the food - borne LM detached had pathogenicity.[Conclusion]The findings can provide some scientific data for the assessment of food safety of animal products and identification of Listeria monocytogenes.