关键词：
羊草(Leymus chinensis);幼穗;外殖体;再生植株
摘 要：
采用离体培养方法首次获得羊草体细胞再生植株。其方法要点是 :取羊草幼穗为外植体 ,经 0 .1%Hg Cl2 溶液表面消毒后 ,接种到含 2 m g/L 2 ,4- D的 MS培养基上 ,置于恒温 2 5℃条件下诱导愈伤组织。在加有 1mg/L 2 ,4-D MS培养基上继代 2次后 ,转移到含 1.0 m g/L KT和 0 .5 mg/L NAA的 MS培养基上分化培养得到再生芽。在无激素的基本培养基上获得了生根的试管苗 ,试管苗移栽到温室后生长正常。研究结果还表明 ,尽管从羊草叶片、幼穗和成熟胚在同样培养条件下均能诱导出愈伤组织 ,但只有幼穗愈伤组织能够继续分化出再生植株。羊草试管苗的分化因基因型和外源激素的不同而异。
译 名：
Plant Regeneration of Leymus chinensis Via in Vitro Culture
作 者：
LIU Gong-she,WANG En-hua,LIU Jie *,QI Dong-mei,LI Fang-fang (Institute of Botany, The Chinese Academy of Sciences, Beijing 100093, China)
关键词：
Leymus chinensis; Immature stachys; Explant; Plant regeneration
摘 要：
The regenerated plants were obtained from Leymus chinensis for the first time through tissue culture.Immature-base explants were cultured on Murashige and Skoog's basal medium(MS) supplement- ed with 2 mg/L 2,4-D after surface sterilized with 0.1% HgCl 2 to induce callus at 25℃ constant temperature.After moved to MS medium containing 1mg/L 2,4-D for two times,the compact calluses were selected and transferred to MS basal medium containing 1.0mg/L Kt and 0.5mg/L NAA,which differentiated into shoot-buds. The shoot-buds appeared and regenerated new roots,finally developed plantlets on 2,4-D-free basal medium. Regenerated plants growth well when transferred into greenhouse.A comparative study of different explants,in this study,revealed that only could the callus from immature stachys differentiate into regenerated plants. The differentiate potential of regenerated plants has close related to the genotype and external hormone.
