中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 大豆SUMO系统相关基因定位及其与细菌性斑点病抗性关系 中国油料作物学报 2015,37 (5) 622-629
Position: Home > Articles > Location of soybean SUMO system related genes and defence to Pseudomonas syringae pv. glycinea Chinese Journal of Oil Crop Sciences 2015,37 (5) 622-629

大豆SUMO系统相关基因定位及其与细菌性斑点病抗性关系

作  者：
林美静;张丽伟;张慧
关键词：
大豆;细菌性斑点病;SUMO;表达
摘  要：
类泛素的小蛋白修饰(small ubiquitin-like modifier,SUMO)对植物抗病防御起重要作用。本研究利用生物信息学方法,确定了大豆SUMO系统6个相关基因。对细菌性斑点病抗病品种黑农37及感病品种合丰25接种处理后各基因表达变化进行实时定量分析。结果发现:抗病品种中,Gm SUMO2、Gm SCEa在叶中24h表达量最高,Gm SUMO3、Gm SCEb在叶中48h表达量最高,所以Gm SUMO2/3和Gm SCEa/b在大豆叶部抗病过程中起重要作用。Gm SAE2a在茎中48h的表达量高达6倍多,在茎部抗病过程中起主要作用。Gm ESD4e在抗病品种的根和叶中表达量快速下降,起着主要的去SUMO化作用。Gm ESD4a/e在感病品种接菌前期和后期分别在叶和根部相对表达量较高,主导感病品种叶和根部的去SUMO化作用,初步说明大豆SUMO系统与细菌性斑点病抗性相关。
译  名：
Location of soybean SUMO system related genes and defence to Pseudomonas syringae pv. glycinea
作  者：
LIN Mei-jing;ZHANG Li-wei;ZHANG Hui;WANG Jin-hui;LIN Meng-meng;YU Guo-long;YU Ren-jing;LIU Chun-yan;CHEN Qing-shan;Agronomy College,Northeast Agriculture University;Land Reclamation Research and Breeding Centre of Heilongjiang;
关键词：
Glycine max;;Pseudomonas syringae pv.glycinea;;SUMO;;Expression
摘  要：
Sumoylation played an important role in pathogen defense of soybean. By bioinformatics method,6SUMO related genes were obtained from soybean. Expression of these genes were analyzed at transcriptional level by real- time PCR after inoculated with Pseudomonas syringae pv. glycinea in resistant variety HN37( Heinong37) and susceptive variety HF25( Hefeng 25). Results showed that,in HN37 leaf,expressions of Gm SUMO2 and Gm SCEa were the highest at 24 h,Gm SUMO3 and Gm SCEb were at 48 h. Therefore Gm SUMO2 /3 and Gm SCEa / b might have played a major role in leaf disease- resistance process. Expression of Gm SAE2 a at 48 h was 6- fold higher than at 0h which might have played a major role in stem disease- resistance. Expression of Gm ESD4 e decreased significantly after inoculation,which might play a major role of desumoylation in HN37. Expression of Gm ESD4 a / e was the highest at 24 h in leaf and at 72 h in root of HF25 which might have played a major role of desumoylation of HF25.In summary,the soybean SUMO system was associated with resistance to P. syringae pv. glycinea.

相似文章

导出引用
计量
文章访问数: 13
HTML全文浏览量: 0
PDF下载量: 1

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46