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当前位置: 首页 > 文章 > 大肠杆菌对奶牛子宫内膜组织合成分泌IL-1β、IL-6和PGE2的影响 中国兽医学报 2019 (6) 1208-1213
Position: Home > Articles > 大肠杆菌对奶牛子宫内膜组织合成分泌IL-1β、IL-6和PGE2的影响 Chinese Journal of Veterinary Science 2019 (6) 1208-1213

大肠杆菌对奶牛子宫内膜组织合成分泌IL-1β、IL-6和PGE2的影响

作  者：
李婷婷;毛伟;刘博;刘晓兵;曹金山
关键词：
大肠杆菌;奶牛子宫内膜组织;炎症;IL-1β;IL-6;PGE2
摘  要：
基于奶牛子宫内膜组织体外培养方法,分别采用浓度为1*105,1*106 CFU/mL的致病性大肠杆菌处理体外培养的子宫内膜组织12,24 h后用HE染色方法评价组织损伤情况,ELISA法检测组织培养液上清中IL-1β,IL-6和PGE2的分泌变化,RT-qPCR法检测IL-1β和IL-6基因表达变化.结果显示:与对照组相比,大肠杆菌分别处理12,24 h后奶牛子宫内膜组织中子宫内膜上皮细胞和腺上皮细胞脱落、坏死、崩解,并且显著提高奶牛子宫内膜组织IL-1β,IL-6和PGE2的分泌及IL-1β和IL-6的mRNA的表达量.结果表明:成功建立了体外大肠杆菌型奶牛子宫内膜炎模型,且IL-1β,IL-6和PGE2在大肠杆菌引起的奶牛子宫内膜组织炎症反应中发挥着重要的调控作用.
作  者：
LI Ting-ting;MAO Wei;LIU Bo;LIU Xiao-bing;CAO Jin-shan;College of Veterinary Medicine,Inner Mongolia Agricultural University;Key Laboratory of Clinical Diagnosis and Treatment Techniques for Animal Disease,Ministry of Agriculture;
单  位：
LI Ting-ting%MAO Wei%LIU Bo%LIU Xiao-bing%CAO Jin-shan%College of Veterinary Medicine,Inner Mongolia Agricultural University%Key Laboratory of Clinical Diagnosis and Treatment Techniques for Animal Disease,Ministry of Agriculture
关键词：
Escherichia coli;;bovine endometrial tissue;;inflammation;;IL-1β;;IL-6;;PGE_2
摘  要：
Endometrial explants in vitro culture,were stimulated by endometrial pathogenic E.coli(1×10~5,1×10~6 CFU/mL) for 12,24 h,followed by the detection of endometrial explants damage by HE,the section of IL-1β,IL-6 and PGE_2 in the endometrial explants supernatant by ELISA and the differential expression of IL-1β and IL-6 by RT-PCR.The results showed that the histological sections of E.coli induced endometrium indicated that the endometrial glands were not clearly visible,and endometrial epithelial cells and glandular epithelial cells had disappeared after treatment of E.coli for 12,24 h,and the IL-1β,IL-6 and PGE_2 section and IL-1β,IL-6 mRNA expression were significantly up-regulated compared with control group.The results indicated that the in vitro cultivation method of Escherichia coli induced bovine endometrial tissue was successfully established,and IL-1β,IL-6 and PGE_2 may play an important role in the inflammatory response of bovine endometrium induce by E.coli.

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