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当前位置: 首页 > 文章 > Study on the cloning and isolation of sus scrofa GPX2 gene by RACE method. 农业科学与技术(英文) 2008,9 (1) 24-28
Position: Home > Articles > Study on the cloning and isolation of sus scrofa GPX2 gene by RACE method. Agricultural Science & Technology 2008,9 (1) 24-28

Study on the cloning and isolation of sus scrofa GPX2 gene by RACE method.

作  者：
Zhao Hua;Zhou JiChang;Li JunGang;Zhao Ying;Wang KangNing
单  位：
The Center of Future Agriculture for Human Health; China.; Sichuan Agriculture University; Ya'an 625014
关键词：
DNA cloning;genes;isolation;nucleotide sequences;pigs
摘  要：
The study was conducted to clone and isolate the glutathione peroxidase 2 (GPX2) gene of pigs using RACE method. Total RNA from the duodenum were used as template, while degenerated primers were designed according to the homology alignment analysis of GPX2 gene of human, rat, mouse, dog and cattle. A 300 bp sequence of GPX2 gene was obtained using RT-PCR. The gene sequence of GPX2 was analysed. Results revealed that an mRNA sequence of 924 bp was successfully cloned and isolated. The sequence contained complete 3' end and higher sequence homology with GPX2 gene of human, mouse, cattle and dog. It is suggested that sequence alignment analysis identifies the cloned gene as Sus scrofa GPX2 gene (NCBI GenBank database, sequence number DQ98982).

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