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当前位置: 首页 > 文章 > 锶对苜蓿生长和抗氧化酶系统的影响 中国草地学报 2018 (2) 27-33
Position: Home > Articles > Effects of Stronium on Growth and Antioxidative System of Alfalfa Chinese Journal of Grassland 2018 (2) 27-33

锶对苜蓿生长和抗氧化酶系统的影响

作  者：
何庆元;王松华;李正鹏;张晓红;詹秋文
单  位：
安徽科技学院农学院;安徽科技学院生命科学学院
关键词：
苜蓿;锶;抗氧化酶;同工酶
摘  要：
在1/2浓度Hoagland营养液中添加终浓度至0、1mmol/L、5mmol/L、10mmol/L、20mmol/L和40mmol/L的SrCl_2处理2叶1心期苜蓿,待其生长至3叶1心期分析6个处理对苜蓿生长、抗氧化酶(POD、SOD和CAT)活性和同工酶谱的影响。结果表明:SrCl_2处理在低浓度对苜蓿生长有一定促进作用,浓度超过5mmol/L后,苜蓿生长受到抑制。当SrCl_2处理浓度在0~40mmol/L范围内,苜蓿POD、SOD和CAT活性表现为先升高后降低,其中POD和CAT在10mmol/L、SOD在20mmol/L浓度时活性最高。SOD/POD、SOD/CAT和SOD/(POD+CAT)比值先降低后升高,10mmol/L SrCl_2处理比值最小,说明POD和CAT活性在锶胁迫时降低更快,限制了锶胁迫的缓解。锶处理导致POD同工酶谱带减少,20mmol/L SrCl_2可诱导产生2条新的SOD谱带,同时导致SOD-3不表达,10mmol/L SrCl_2诱导1条新的CAT谱带;但随浓度增加,新产生的酶不表达,没有相应谱带。
译  名：
Effects of Stronium on Growth and Antioxidative System of Alfalfa
作  者：
HE Qing-yuan;WANG Song-hua;LI Zheng-peng;ZHANG Xiao-hong;ZHAN Qiu-wen;Life Science College,Anhui Science and Technology University;Agronomy College,Anhui Science and Technology University;
关键词：
Alfalfa;;Strontium;;Antioxidant enzyme;;Isoenzyme
摘  要：
The alfalfa in 2 leaves 1 heart were treated with different SrCl_2 concentration(0.00,1.00,5.00,10.00,20.00 and 40.00 mmol/L)in 1/2-strength Hoagland nutrient solution,and grew to 3 leaves 1 heart.The effects of different treatment on growth traits,enzyme activities and isoenzymes of POD,SOD,CAT were analyzed.The results showed that growth of alfalfa was promoted by low concentration of SrCl_2.The growth of alfalfa was inhibited when the SrCl_2 concentration was more than 5 mmol/L.The activities of antioxidant enzymes(POD,SOD and CAT)were increased first and then decreased when the concentration of SrCl_2 ranged from 0.00 to 40.00 mmol/L,and the activities of POD and CAT were the highest at 10 mmol/L SrCl_2 and SOD at 20 mmol/L SrCl_2.The ratio of SOD/POD,SOD/CAT and SOD/(POD+CAT)decreased first then increased,the ratio was minimal at 10 mmol/L SrCl_2 treatment,it was a limiting factor of alleviation strontium stress,the activities of POD and CAT decreased faster in stress.POD isozyme bands were decreased along with strontium concentration increasing.The 2 new bands were induced and SOD-3 band vanished at 20 mmol/L SrCl_2 treatment,There was 1 new CAT band in10 mmol/L SrCl_2 treatment.However,the new isoenzymes were not expressed and there was no corresponding band with the increase of concentration.These new isozymes may be the key pathway to resist the stress of strontium.

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