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当前位置: 首页 > 文章 > 长期施肥对土nirS型反硝化细菌的影响及其与N_2O排放的关系 水土保持研究 2022 (2) 99-105
Position: Home > Articles > Effect of Long-Term Fertilization on nirS-Type Denitrifer and Its Relationship with N_2O Emission in Eum-Orthic Anthrosols Research of Soil and Water Conservation 2022 (2) 99-105

长期施肥对土nirS型反硝化细菌的影响及其与N_2O排放的关系

作  者：
刘耕苑;肖杰;高明霞;孙本华;张树兰;杨学云;冯浩;张彤勋
单  位：
中国旱区节水农业研究院;西北农林科技大学资源环境学院/农业部西北植物营养与农业环境重点实验室;西北农林科技大学水土保持研究所;西北农林科技大学水利与建筑工程学院
关键词：
长期施肥;nirS型反硝化细菌;氧化亚氮;实时定量PCR;高通量测序
摘  要：
土壤反硝化作用是土壤N_2O产生的重要过程,亚硝酸盐还原酶(NIR)催化的亚硝态氮(NO~-_2)还原为一氧化氮(NO)是反硝化作用的关键环节,研究长期施肥对反硝化微生物的影响及其与N_2O排放的关系对于全面理解土壤反硝化过程具有重要意义。基于28年的旱作雨养长期施肥试验,通过常规监测、定量PCR和高通量测序等探讨了长期不同施肥(不施肥CK、偏施肥的单施氮肥N和氮钾配施NK、以及氮磷钾平衡施肥NPK)下■土N_2O排放和nirS反硝化细菌群落特征及两者之间的关系。结果表明:长期化肥施用(N,NK和NPK)均显著提高了N_2O累积排放量,其中平衡施肥(NPK)最高。长期化肥施用对nirS基因丰度和nirS型反硝化细菌的α-多样性无显著影响,但长期平衡施用化肥提高了uncultured_bacterium_2303和Rhodanobacter_sp._D206a的相对丰度,降低了unclassified_k_norank_d_Bacteria和unclassified_p_Proteobacteria的相对丰度,从而改变了nirS型反硝化细菌的群落结构组成。雨养旱作条件下,土壤有机碳(SOC)、全氮(TN)、有效磷(AP)和pH等土壤性质是■土nirS型反硝化细菌群落结构组成变化的主要影响因素。■土nirS型反硝化细菌群落结构组成对土壤N_2O排放具有显著影响,而nirS基因丰度和nirS型反硝化细菌多样性并没有显著影响。
译  名：
Effect of Long-Term Fertilization on nirS-Type Denitrifer and Its Relationship with N_2O Emission in Eum-Orthic Anthrosols
作  者：
LIU Gengyuan;XIAO Jie;GAO Mingxia;SUN Benhua;ZHANG Shulan;YANG Xueyun;FENG Hao;ZHANG Tongxun;College of Natural Resources and Environment, Northwest A&F University/Key Laboratory of Plant Nutrition and Agro-Environment, Ministry of Agriculture;College of Water Resources and Architectural Engineering, Northwest A&F University;Institute of Water Saving Agriculture in Arid Region of China;Institute of Soil and Water Conservation, Northwest A&F University;
关键词：
long-term fertilization;;nirS-type denitrifier;;nitrous oxide;;quantitative real-time PCR;;high-throughput sequencing
摘  要：
Soil denitrification is an important process for the production of soil N_2O, and that Nitrite reductase(NIR) catalyzes the reduction of nitrite nitrogen(NO~-_2) to nitric oxide(NO) is the key step of denitrification. The study on the impact of long-term fertilization on denitrifying microorganisms and its relationship with soil N_2O emissions is of great significance for a comprehensive understanding of the soil denitrification process. Based on 28 years of long-term fertilization experiments in rain-fed dry farming system, the characteristics of soil nirS-type denitrifying bacterial communities and N_2O emission and their relationship were investigated under different types of long-term fertilization(No fertilization of CK, only N fertilization and NK combined fertilization of partial fertilizations, and balanced fertilization of NPK) by conventional monitoring, quantitative PCR and high-throughput sequencing. The results showed that all types of long-term fertilization(N, NK and NPK) significantly increased the cumulative N_2O emission(EN_2O), in which balanced fertilization(NPK) caused the the highest cumulative N_2O emission; long-term fertilization had no significant effect on nirS gene abundance and the α-diversity of nirS-type denitrifying bacteria, while long-term balanced fertilization increased the relative abundance of uncultured_bacterium_2303 and Rhodanobacter_sp._D206 a, and reduced the relative abundance of unclassified_k_norank_d_Bacteria and unclassified_p_Proteobacteria, thereby changed the community structure of nirS-type denitrifying bacteria. Soil properties such as soil organic carbon, total nitrogen, available phosphorusand pH are the main factors influencing the nirS-type denitrifying bacterial community structure that has a significant effect on soil N_2O emission, while the abundance of nirS gene abundance and the diversity of nirS-type denitrifying bacteria have no significant effects in Eum-Orthic Anthrosols under rain-fed dry farming system.

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