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Position: Home > Articles > A Study on SRAP Markers for Nucleus Male Sterile Genes in Sugar Beet Acta Agriculturae Boreali-Sinica 2012,27 (1) 109-112

甜菜雄性核不育基因的SRAP标记

作  者:
伊六喜;斯钦巴特尔;孟显光;赵瑞哗;张惠忠;田自华
单  位:
内蒙古农牧业科学院;内蒙古农业大学农学院
关键词:
甜菜;雄性核不育;SRAP标记
摘  要:
采用SRAP技术对甜菜核雄性不育基因进行分子标记的研究,为甜菜育种提供了一条新的途径。以遗传背景相似的可育株和不育株甜菜为材料,用64对SRAP引物组,进行了SRAP分子标记研究。在64对引物组中有2对引物组(即M2E7和M8E8)可分别得到1个育性基因相关的SRAP分子标记,即M2E7-480和M8E8-130,出现频率分别为73%和76%。表明该SRAP标记技术可用于甜菜辅助育种及遗传多样性分析,从而为甜菜雄性不育相关基因的表达提供科学依据。
译  名:
A Study on SRAP Markers for Nucleus Male Sterile Genes in Sugar Beet
作  者:
YI Liu-xi1,SI Qin-ba-teer 1,MENG Xian-guang2,ZHAO Rui-hua2,ZHANG Hui-zhong2,TIAN Zi-hua1(1.Institute of Agronomy,Inner Mongolia Agriculture University,Huhhot 010019,China;2.Inner Mongolia Academy of Agriculture and Husbandry Sciences,Huhhot 010031,China)
关键词:
Sugar beet;Nuclear male sterility;SRAP markers
摘  要:
A study on SRAP molecular markers for nuclear male sterile genes in sugarbeet was carried out,using male sterile plants and fertile plants with similar genetic background.Results:two pairs of SRAP primers,namely M2E7 and M8E8,produced stable differential PCR bands with frequency of 73% and 76% respectively,which are related to fertility gene.Conclution:this SRAP technque could be used in sugarbeet breeding,genetic diversity studies and research on genes related to fertility in sugarbeet.

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