关键词：
七重PCR;羊肉产品;动物源性成分;种属鉴别
摘 要：
试验建立了猪、牛、绵羊、山羊、鸡、马和牦牛种属鉴别的七重PCR体系,分析了牛、牦牛、山羊源性成分七重PCR检测的灵敏度,检测了10种市售羊肉产品中的动物源性成分。结果表明,采用常规的琼脂糖凝胶电泳可以区分157～517 bp的片段及相互差异在41 bp以上的多重PCR扩增产物,从而实现对这7个物种的快速及准确鉴别,其中对3个物种(牛、牦牛、山羊)DNA的检测灵敏度在2.5 ng左右;所检测的10种羊肉产品中有2种并不是包装上所宣称的羊肉,而是混杂有牛肉或完全用牛肉替代。
译 名：
Application of Septenary Multiplex PCR Detection for Some Animal Components in Sheep and Goat Meat Products
作 者：
FENG Hai-yong1,2,HAN Jian-lin1,2(1.Key Laboratory of Domestic Animal Genetic Resources and Germplsam Innovation of Ministry of Agriculture,Institute of Animal Sciences,Chinese Academy of Agricultural Sciences(CAAS),Beijing 100193,China;2.CAAS-ILRI joint Laboratory on Livestock and Forage Genetic Resources,Institute of Animal Sciences,Chinese Academy of Agricultural Sciences(CAAS),Beijing 100193,China)
关键词：
sepenary multiplex PCR;sheep and goat meat product;animal origin;species identification
摘 要：
Septenary multiplex PCR method and protocol to identify DNAs of pig,cow,sheep,goat,chicken,horse and yak was optimized and developed.Sensitivity of the three species(cow,yak and goat) in the septenary multiplex PCR system was analysed.10 products made of sheep and goat meat were detected.The results showed that routine agarose gel electrophoresis was sufficient to differentiate the multiplex PCR products ranging from 175 to 517 bp and differing by at least 41 bp each other,indicating that this septenary multiplex PCR method could be used for rapid and accurate identification of the seven species and for three species(cow,yak and goat) at sensitivity of 2.5 ng DNA.Among the 10 products examined using this protocol,2 products were found to have mixed with some beef or completely adulterated by beef.
