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Position: Home > Articles > Rapid Analysis of Clorsulon Residue in Milk with High Performance Liquid Chromatography-Tandem Mass Spectrometry FOOD SCIENCE 2008,29 (12) 638-640

液相色谱-质谱/质谱法快速测定牛奶中的氯舒隆残留量

作  者:
郭俊峰;马昕;尚楠
单  位:
宁夏出入境检验检疫局技术中心;河南出入境检验检疫局技术中心;中国农业大学食品科学与营养工程学院
关键词:
牛奶;氯舒隆;Quechers;液相色谱-质谱/质谱
摘  要:
建立快速测定牛奶中氯舒隆残留量的液相色谱-质谱/质谱(LC-MS/MS)检测方法。样品用乙腈提取,Quechers方法净化后,采用LC-MS/MS电喷雾负离子(ESI-)电离,多反应监测(MRM)模式检测,外标法定量。方法定量限为5μg/kg。在添加水平为5~100μg/kg的范围内,氯舒隆的回收率为92.8%~99.3%;相对标准偏差(RSD)不高于4.7%。
译  名:
Rapid Analysis of Clorsulon Residue in Milk with High Performance Liquid Chromatography-Tandem Mass Spectrometry
作  者:
GUO Jun-feng1,MA Xin2,SHANG Nan3 (1. Technical Center of Henan Entry-Exit Inspection and Quarantine Bureau,Zhengzhou 450003,China; 2. Technical Center of Ningxia Entry-Exit Inspection and Quarantine Bureau,Yinchuan 750000,China; 3.College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083,China)
关键词:
milk;clorsulon;Quechers;high performance liquid chromatography-tandem mass spectrometry(LC-MS/MS)
摘  要:
An effective method was developed to determine clorsulon in milk with high performance liquid chromatography-tandem mass spectrometry(LC-MS/MS). Samples were extracted with acetonitrile.The concentrated extracts were cleaned up though Quechers method. Identification was achieved in electrospray ionizationin negative mode(ESI-) using multiple reaction monitoring(MRM).The limit of quantification for clorsulon is 5 μg/kg.The recoveries of clorsulon are 92.8% to 99.3% at spiked levels of 5 to 100 μg/kg.The(RSD) of this relative standard deviation method is less than 8.6%.

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