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Position: Home > Articles > Cloning and Sequence Analysis of Cinnamoyl-CoA Reductase Gene from Guadua paraguayana Journal of Bamboo Research 2016,35 (2) 10-15

巴拉圭瓜多竹CCR基因的克隆与分析

作者：

朱金鑫;孙金金;原晓龙;王娟;杨宇明;王毅

单位：

国家林业局重点开放性实验室云南珍稀濒特森林植物保护和繁育实验室

关键词：

瓜多竹;木质素生物合成;肉桂酰辅酶A还原酶;基因克隆;荧光定量PCR

摘要：

肉桂酰辅酶A还原酶(cinnamoyl-Co A reductase,CCR)是木质素生物合成途径中关键酶。本研究依据转录组数据设计特异性引物,采用RT-PCR方法成功地从巴拉圭瓜多竹(Guadua paraguayanan)中克隆得到一个全新的CCR基因的全长cDNA序列,命名为GpCCR。序列分析结果表明,GpCCR包含完整的c DNA开放阅读框(ORF),由1065bp组成,编码354个氨基酸。Blast比对结果显示该蛋白质属于CCR家族蛋白;系统进化树结果显示瓜多竹与禾本科植物大麦、水稻等亲缘关系较近。荧光定量PCR检测显示GpCCR在巴拉圭瓜多竹的茎秆中表达量最高,在叶中表达量最低。

译名：

Cloning and Sequence Analysis of Cinnamoyl-CoA Reductase Gene from Guadua paraguayana

作者：

ZHU Jin-xin;SUN Jin-jin;YUAN Xiao-long;WANG Juan;YANG Yu-ming;WANG Yi;Yunnan Laboratory for Conservation of Rare,Endangered & Endemic Forest Plants,Public Key Laboratory of the State Forestry Administration; Yunnan Provincial Key Laboratory of Cultivation and Exploitation of Forest Plants;Southwest Forestry University;Yunnan Forestry Academy;

关键词：

Guadua paraguayanan;;Lignin biosynthesis;;Cinnamoyl-coA reductase;;Gene clone;;Real time PCR

摘要：

Cinnamoyl-co A reductase( CCR) is an important enzyme in the biosynthetic pathway of lignin. GpCCR was successfully obtained from Guadua paraguayanan by RT-PCR with the primers specially designed based on the data from transcriptome. Sequence analysis showed the GpCCR had complete c DNA open readimg frame( ORF),which consisted of1065 bp encoding 354 amino acids. Blast analysis showed that GPCCR belonged to the CCR family. Phylogenetic analysis showed that the Guadua paraguayanan had closer relative relationship with Hordeum vulgare and Oryza sativa in the Gramineae. Real time PCR detection showed GpCCR was expressed the strongest in culms and the lowest in leaves of Guadua paraguayana.

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巴拉圭瓜多竹CCR基因的克隆与分析

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