中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 东海带鱼DNA条形码的建立及COⅠ序列变异分析 浙江海洋学院学报(自然科学版) 2013,32 (1) 10-13
Position: Home > Articles > DNA Barcoding Construction and Genetic Diversity Revealed by mtDNA COⅠ of Trichiurus lepturus in the East China Sea Journal of Zhejiang Ocean University(Natural Science) 2013,32 (1) 10-13

东海带鱼DNA条形码的建立及COⅠ序列变异分析

作  者：
李鹏飞;朱文斌;贺舟挺;徐开达;薛利建;张洪亮
单  位：
浙江海洋学院海洋与渔业研究所浙江省海洋水产研究所
关键词：
东海;带鱼;DNA条形编码;COⅠ;遗传多样性
摘  要：
采用聚合酶链反应(PCR)技术对浙江近海水域带鱼群体(n=15)的mtDNA COⅠ基因序列进行扩增,获得了大小约为650 bp的扩增产物。对PCR产物进行双向测序,得到了609 bp的基因片段(除去两端的部分序列),获得了带鱼的DNA条形编码。用DNAMAN软件进行了排序比较发现,东海带鱼的COⅠ基因序列同源性高达99.69%;用DNASP软件分析得知,15个序列共有10个单倍型(在GenBank登录号:GU970070-GU970075,GU970079-GU970082),在15个个体中,共检测到13个变异位点,包括11个转换和2个颠换位点。运用MEGA软件计算出了不同个体间的遗传距离,并据此构建了UPGMA和NJ系统树。用DNASP软件计算出的多态位点数为13、核苷酸多样性指数和平均核苷酸差异数分别为0.005 13和3.124。研究结果表明,东海带鱼的COI基因个体变异程度比较小,对研究带鱼的种群结构和不同地理种群的遗传分化具有一定的应用价值。
译  名：
DNA Barcoding Construction and Genetic Diversity Revealed by mtDNA COⅠ of Trichiurus lepturus in the East China Sea
作  者：
LI Peng-fei,ZHU Wen-bin,HE Zhou-ting,et al(Marine and Fishery Research Institute of Zhjiang Ocean University,Marine Fisheries Research Institute of Zhejiang Province,Zhoushan 316100,China)
关键词：
East China Sea;Trichiurus lepturus;DNA Barcoding;COⅠ;genetic diversity
摘  要：
The PCR technique was used to amplify the mtDNA COⅠgene in 15 individuals of Trichiurus lepturus collected from the East China Sea,along the offing sea area of Zhejiang.The PCR products were sequenced and about 650 bp of COⅠgene were obtained.Remove partial sequences of the two ends 609 bp sequences were obtained,regarding as the DNA Barcoding of T.lepturus.By using DNAMAN to align and compare the sequences of these 15 individuals with each other,the identity of CO Ⅰpairwise sequences was 99.69 % and by using DNASP to analyze the genetic diversity,the result showed that there were 10 haplotypes(GenBank accession no.GU970070-GU970075,GU970079-GU970082) and 13 variation sites were observed,of which there were 11 transition sites and 2 transversion sites,the number of polymorphic sites,the nucleotide diversity and the average number of nucleotide defferences were calculated simultaneous and the results were 13,0.005 13 and 3.124,respectively.The pairwise genetic distances were computed by MEGA.The UPGMA and NJ phylogenetic trees were obtained through the cluster analysis over the pairwise genetic distance.All the results showed that the genetic diversity in CO Ⅰ gene of T.lepturus in the East China Sea was relatively low and proviede a basic molecular biology clue to the studies on population genetics and COⅠgene can be one of the genetic markers available for studing groups structure.

相似文章

导出引用
计量
文章访问数: 12
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46