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Position: Home > Articles > Analysis of Azadirachtin EC by Solvent Extraction-HPLC Agrochemicals 2018 (2) 114-116+132

溶剂萃取-高效液相色谱法分析印楝素乳油中印楝素A含量

作者：

许柏球;王一珊;翁盼伟;王金林;周靖;崔淑芬

单位：

南昌大学生命科学学院;深圳职业技术学院应用化学与生物技术学院;广东省荔枝龙眼病虫防控科技创新中心

关键词：

印楝素乳油;印楝素A;HPLC-DAD;溶剂萃取;峰纯度

摘要：

[目的]建立快速测定印楝素乳油中印楝素A含量的分析方法。[方法]用石油醚萃取杂质,以5 mmol/L乙酸铵水溶液+乙腈(体积比40∶60)为流动相,检测波长为218 nm,采用等质量浓度洗脱的方法进行HPLC-DAD分析。[结果]该方法在5~200 mg/L范围内呈良好的线性关系,相关系数(R~2)大于0.999,最低检出质量浓度(LOD)为0.5 mg/L,定量限(LOQ)为1.3 mg/L,相对标准偏差(RSD)为2.87%,加标回收率为99.53%~102.32%。[结论]该方法分离度高,准确度和精密度好,分析速度快,适合印楝素乳油产品中印楝素A含量分析。

译名：

Analysis of Azadirachtin EC by Solvent Extraction-HPLC

作者：

XU Bai-qiu;WANG Yi-shan;WENG Pan-wei;WANG Jin-lin;ZHOU Jing;CUI Shu-fen;School of Applied Chemistry and biotechnology,Shenzhen Polytechnic;Guangdong Litchi Longan Pest Control Science and Technology Innovation Center;Department of Bioscience, Nanchang University;

关键词：

azadirachtin EC;;azadirachtin A;;HPLC-DAD;;solvent extraction;;peak purity

摘要：

[Aims] This study aims to establish a rapid method for the determination of azadirachtin A in azadirachtin emulsifiable concentrates(azadirachtin EC). [Methods] The pretreatment of azadirachtin EC samples was carried out by removal of impurities with petroleum ether extraction. Azadirachtin A was quantified by using HPLC coupled with the diode array detection(DAD). The HPLC separation was performed with the isocratic gradient elution using 5 mmol/L ammonium acetate solution and acetonitrile(40:60, by vol) as the mobile phase. The wavelength for the DAD was 218 nm. [Results] The method showed excellent performance when the calibration curve was linear over the range of 5-200 mg/L with the correlation coefficient R~2>0.999. The limit of detection(LOD) and the limit of quantitation(LOQ) were 0.5 and 1.3 mg/L, respectively. The relative standard deviation(RSD) was 2.87% and the recovery rate was 99.53-102.32%. [Conclusions] Since the method is simple and quick while showing high resolution,accuracy, and precision, it is thus suitable for the determination of azadirachtin A in azadirachtin EC.

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溶剂萃取-高效液相色谱法分析印楝素乳油中印楝素A含量

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