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当前位置: 首页 > 文章 > 藏系绵羊VEGF基因的克隆、同源性分析及其表达水平的实时荧光定量PCR检测 畜牧与兽医 2011,43 (10) 25-30
Position: Home > Articles > Cloning,homology analysis and expression level of the VEGF gene by a real-time fluorescent quantitative PCR in Tibetan sheep Animal Husbandry & Veterinary Medicine 2011,43 (10) 25-30

藏系绵羊VEGF基因的克隆、同源性分析及其表达水平的实时荧光定量PCR检测

作  者：
窦全林;杨发龙;白文林;马志杰;尹荣焕;陈刚
单  位：
青海大学畜牧兽医科学院;沈阳农业大学畜牧兽医学院;西南民族大学生命科学与技术学院;青海大学农牧学院动物医学系
关键词：
藏系绵羊;VEGF基因;克隆;同源性分析;实时荧光定量PCR
摘  要：
采用PCR技术从藏系绵羊皮肤组织细胞中扩增出了血管内皮细胞生长因子(VEGF)基因,长度为573 bp,共编码190个氨基酸;序列比对发现,VEGF基因在所选择的多个物种之间具有高度同源性,相似性在100%～74.5%之间,其中藏系绵羊与普通绵羊的VEGF基因序列完全相同,其次为牛和猪,相似性分别为99.1%和96.2%,而与鸡的同源性最低,相似性为74.5%。采用实时荧光定量PCR SYBR Green I荧光染料法,对藏系绵羊皮肤组织细胞中VEGF基因的表达水平进行了相对定量分析,建立了快速检测VEGF基因表达量的方法。检测结果表明:给妊娠后期藏系母羊补饲自制的营养舔砖和颗粒饲料后,所产羔羊体内VEGF基因的表达水平分别为对照组的11.5倍和0.85倍。为提高羔羊皮肤组织中VEGF基因mRNA的表达水平,对妊娠后期藏系母羊补饲自制的营养舔砖,效果优于补饲颗粒饲料。
译  名：
Cloning,homology analysis and expression level of the VEGF gene by a real-time fluorescent quantitative PCR in Tibetan sheep
作  者：
DOU Quan-lin1,YANG Fa-long2,BAI Wen-lin3,MA Zhi-jie4,YIN Rong-huan3,CHEN Gang1(1.College of Agriculture and Animal Science,Qinghai University,Xining 810016,China; 2.College of Life Science and Technology,Southwest University for Nationalities,Chengdu 610041,China; 3.College of Animal Science and Veterinary Medicine,Shenyang Agricultural University,Shenyang 110161,China; 4.Academy of Animal Science and Veterinary Medicine,Qinghai University,Xining 810016,China)
关键词：
Tibetan sheep;VEGF gene;clone;homology analysis;real time fluorescent quantitative PCR
摘  要：
The VEGF gene was amplified from the skin of Tibetan sheep by PCR,573 bp in length and coded 190 amino acids.Our results indicated that the homology of VEGF gene between Tibetan sheep and some other species was from 100% to 74.5%,in which the similarity of the gene sequence of Tibetan sheep and ordinary sheep was 100%,99.1% and 96.2% similar to cattle and pigs,but compared with chicken,the homology was 74.5%.The expression level of VEGF gene from Tibet sheep skin cells were analyzed by using fluorescence quantitative PCR SYBR Green I Fluorescent dyes technology.The results indicated that the expression level of VEGF gene on lamb was 11.5 and 0.85 times,respectively in the Tibetan pregnancy female sheep groups fed with Nutrition Brick and Granule Feed compared with that of control group.To improve the expression level of VEGF gene in the lamb,it is better to feed Nutrition Brick to Tibetan pregnancy female sheep than Granule Feed.

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