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当前位置: 首页 > 文章 > 小麦EST-SSR的分析及其引物的开发 云南农业大学学报(自然科学) 2012,27 (5) 623-630
Position: Home > Articles > Analysis and Exploitation of EST-SSRs in the Triticum aestivum Journal of Yunnan Agricultural University(Natural Science) 2012,27 (5) 623-630

小麦EST-SSR的分析及其引物的开发

作  者：
刘林;孙来亮;兰茗清;杨静;查友贵;李成云
单  位：
农业生物多样性与病虫害控制教育部重点实验室;云南农业大学植物保护学院;农业生物多样性应用技术国家工程研究中心
关键词：
小麦;表达序列标签;微卫星
摘  要：
为开发出具有丰富多态性的、新型的小麦EST-SSR标记,利用已公布的1 071 367条小麦表达序列标签(expressed sequence tags,EST)序列,对≥24 bp的微卫星或简单重复序列进行了系统分析。结果表明,在所分析的小麦EST序列中,搜索到长度大于或等于24 bp,基序匹配值大于80%的SSR序列32 350个。在这些SSR中,单碱基SSR最多,数量达18 075个,占SSR总数的55.9%;其次为3核苷酸SSR,共6 106个,占SSR总数的18.7%,重复数大于30次以上的达444个,占所有3核苷酸SSR的7.2%。4核苷酸SSR数量最少,仅有696个,仅占SSR总数的2.2%。研究中收搜索到的6核苷酸SSR共1 562个,其基序组成可分为145类,优势基序为AGCCGC(达214个)。以上结果说明小麦EST序列中含有丰富的SSR,这非常有利于开发多态性较高的EST-SSR标记。
译  名：
Analysis and Exploitation of EST-SSRs in the Triticum aestivum
作  者：
LIU Lin,SUN Lai-liang,LAN Ming-qing,YANG Jing,ZHA You-gui,LI Cheng-yun(Key Laboratory of Agro-Biodiversity and Pest Management of Education Ministry of China;National Engineering Research Center Agro-Biodiversity Applied Technology;College of Plant Protection, Yunnan Agricultural University,Kunming 650201,China)
关键词：
wheat(Triticum aestivum);expressed sequence tags(EST);simple sequence repeat(SSR)
摘  要：
A total of 1 071 367 expressed sequence tags(ESTs) of Triticum aestivum was searched for mono-to hexa-nucleotide simple sequence repeats(SSR or microsatellite) using software of "Tandem Repeats Finder".And the type,size and frequency of these SSRs were determined.The results showed that a total of 32 350 SSRs were found out in these ESTs with the criteria of SSR length ≥24 bp and 80% matches.Considering all six classes of SSR,the most abundant microsatellite was mono-nucleotide repeat,and the number of mono-nucleotide repeat SSR was 18 075,occupied 55.9% of total SSRs,followed by tri-nucleotide repeat,which number was 6 106,accounting for 18.7% of all SSRs,and there were 444 tri-nucleotide repeat SSRs whose repeat number was more than 30,accounting for 7.2%.The least microsatellites were tetra-nucleotide,their number was 696,only accounting for 2.2% of all SSRs.Especially,1 562 hexa-nucleotide repeats SSRs were observed,and their motifs were also very abundance,with 145 types,and AGCCGC is the major motif,being 214.In this study,because the size of all SSRs was more than or equal to 24 bp,these results would be useful for further molecular biological studies and development of new EST-SSR molecular markers of T.aestivum.

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