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当前位置: 首页 > 文章 > 水稻条纹叶枯病细胞病理变化的观察 植物病理学报 2000,30 (4) 306-311
Position: Home > Articles > CYTOPATHOLOGICAL OBSERVATION OF RICE STRIPE Acta Phytopathologica Sinica 2000,30 (4) 306-311

水稻条纹叶枯病细胞病理变化的观察

作  者：
刘利华;吴祖建;林奇英;谢联辉
单  位：
福建农业大学植物病毒研究所;福州35
关键词：
水稻条纹病毒;免疫胶体金标记;细胞病理变化
摘  要：
利用透射电子显微镜和免疫胶体金标记技术 ,观察水稻条纹叶枯病病叶细胞病理变化 ,病毒粒体、病毒外壳蛋白和病害特异蛋白在寄主细胞中的分布。结果表明 ,病叶叶肉细胞中线粒体增多 ,细胞核变大 ,几乎每个细胞中都有蛋白体 ,叶绿体基粒片层变稀疏和变粗 ,或被挤压和变细 ,或叶绿体解体 ,基粒片层残留于细胞质中。在叶肉细胞和维管束筛管细胞中发现一些内含体和电子密度较高的物体 ,在高放大倍数下 ,可以清楚的看出为点状和丝状结构 ,其直径约为 8nm。免疫胶体金标记显示 ,病毒外壳蛋白和病害特异蛋白存在于叶绿体、细胞质和细胞核以及细胞壁上 ,但线粒体中没有发现
译  名：
CYTOPATHOLOGICAL OBSERVATION OF RICE STRIPE
作  者：
LIU Li hua, WU Zu jian, LIN Qi ying, XIE Lian hui (Institute of Plant Virology, Fujian Agricultural University, Key Laboratory of Plant Virology of Fujian Province, Fuzhou 350002, China)
关键词：
Rice stripe tenuivirus; immunogoldlabel; cytopathological alteration
摘  要：
By electron microscope and immunogold labeling technique, the cytopathological alteration of rice stripe virus infected leaves, and the distribution of the virus particles, the coat protein and the disease specific protein in the cells were observed. In mesophyll cell, the quantity of the mitochondria increased and the nuclear enlarged. There were protein bodies in most of mesophyll cells. The ultrastructure of chloroplast granum of the disease leaves was altered. Its granum appeared sparse and thick, or was forced tightly and became thin. In some cases the chloroplast was destroyed, while the rested granum remained in the cytoplasm. In the mesophyll and sieves in vascular bundle of the disease leaves, there were some inclusions and bodies which showed higher electronic density. With higher amplification they were dots and filaments about 8 nm in diameter. The coat proteins and the disease specific protein were found in the chloroplast, cytoplasm and nuclear, but not in mitochondria.

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