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Position: Home > Articles > Isolation and Characterization of Hemicelluloses from Bamboo(Dendrocalamus giganteus Munro) Acta Agriculturae Boreali-occidentalis Sinica 2014,23 (5) 195-201

龙竹半纤维素的提取分离及结构表征

作  者:
肖霄;李居南;王志文;於祥;徐晖
单  位:
西北农林科技大学理学院;中粮营养健康研究院品牌食品研发中心;西北农林科技大学林学院
关键词:
半纤维素;二甲亚砜;结构表征;核磁共振
摘  要:
龙竹竹粉经脱蜡及脱淀粉后,再经亚氯酸钠脱木素制得综纤维素,进一步用二甲亚砜(DMSO)以及KOH溶液提取,经乙醇沉淀后制得半纤维素H1和H2,得率分别为18.7%和39.8%。对半纤维素组分H1和H2进行糖分析、红外光谱以及核磁共振分析。结果表明,龙竹半纤维素为乙酰化的L-阿拉伯糖基-4-O-甲基葡萄糖醛酸木聚糖,其中阿拉伯糖连接在木聚糖主链的C-3位,4-O-甲基葡萄糖醛酸连接在木聚糖主链的C-2位,而乙酰基连接在木聚糖主链的C-2位或C-3位。
译  名:
Isolation and Characterization of Hemicelluloses from Bamboo(Dendrocalamus giganteus Munro)
作  者:
XIAO Xiao;LI Junan;WANG Zhiwen;YU Xiang;XU Hui;College of Science,Northwest A&F University;Food R&D Centre,COFCO Nutrition & Health Research Institute;College of Forestry,Northwest A&F University;
关键词:
Hemicelluloses;;Dimethyl sulfoxide;;Structural characterization;;NMR
摘  要:
Bamboo(Dendrocalamus giganteus Munro)powder was dewaxed with toluene-ethanol and destarched with hot water.The extractive free sample was delignified by NaClO2and then extracted with DMSO and KOH,yielding 18.7%(H1)and 39.8%(H2)hemicellulosic fractions,respectively. Chemical composition and structural characterization of these hemicellulosic fractions were investigated by a combination of HPAEC,FT-IR,1 H-,13C-and HSQC NMR techniques.The result revealed that hemicelluloses from bamboo(Dendrocalamus giganteus Munro)were assumed to L-arabino-4-Omethyl-D-glucurono-D-xylan with arabinose linked on position 3of xylan,4-O-methylglucuronic acid residues linked on position 2of the xylan,and acetyl groups linked on position 2or 3.

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