Position: Home > Articles > Study on icotinib of the proliferation and apoptosis of neurofibromatosis type 2 related schwannoma cells
Journal of Northeast Agricultural University
2015
(7)
57-61
盐酸埃克替尼诱导NF2神经鞘瘤细胞增殖和凋亡研究
作 者：
汪颖;赵赋;张晶;王博;张琪;孙异临;刘丕楠
单 位：
首都医科大学北京神经外科研究所;首都医科大学附属北京天坛医院
关键词：
盐酸埃克替尼;2型神经纤维瘤病;神经鞘瘤;表皮生长因子受体
摘 要：
探讨盐酸埃克替尼对2型神经纤维瘤病(NF2)神经鞘瘤细胞增殖和凋亡的影响。运用0~80μmol·L-1盐酸埃克替尼处理NF2听神经鞘瘤原代细胞及大鼠神经鞘瘤细胞系RT4,孵育48 h后通过CCK8比色法检测药物对细胞增殖活力的影响;流式细胞仪检测药物对细胞凋亡的影响;电子显微镜观察药物处理后细胞显微结构变化;同时应用免疫印迹法及实时定量PCR观察凋亡相关蛋白表达情况。盐酸埃克替尼作用后,细胞增殖活性显著降低,呈剂量依赖性。流式细胞仪检测,10、20、40、80μmol·L-1盐酸埃克替尼作用48 h后,凋亡率分别为(6.10±0.35)%、(10.52±0.87)%、(11.78±0.63)%、(16.05±1.02)%。免疫印迹检测发现,半胱氨酸蛋白水解酶3(Caspase-3)剪切体增多,实时定量PCR结果显示bcl-2基因表达降低,bax基因表达增加。结果表明,盐酸埃克替尼能通过活化Caspase-3及调控bcl-2、bax基因表达诱导细胞凋亡而抑制神经鞘瘤细胞增殖。
译 名：
Study on icotinib of the proliferation and apoptosis of neurofibromatosis type 2 related schwannoma cells
作 者：
WANG Ying;ZHAO Fu;ZHANG Jing;WANG Bo;ZHANG Qi;SUN Yilin;LIU Pinan;Beijing Neurosurgical Institute, Capital Medical University;Beijing Tiantan Hospital, Affiliated of Capital Medical University;
关键词：
icotinib;;neurofibromatosis type 2;;schwannoma;;EGFR
摘 要：
To explore the role of icotinib on the proliferation and apoptosis of neurofibromatosis type 2(NF2) related schwannoma cells. Primary cultured NF2 related human schwannoma cells and rat schwannoma cell line RT4 cells were treated in vitro with icotinib at a concentration gradient of 0-80 μmol· L-1,and its role on the cell proliferation and apoptosis were analyzed by the CCK8 assay and flow cytometer,respectively. The ultrastructural changes was evaluated by electron microscopy, and apoptosis- related proteins and genes were detected using Western- blot and RT- PCR. Results showed that icotinib inhibites the proliferation of primary NF2-related schwannoma cells and RT4 cells in a dose dependent manner, after treatment with 10, 20, 40 and 80 μmol· L- 1icotinib for 48 h, the rates of cell apoptosis were(6.10±0.35)%,(10.52 ± 0.87)%,(11.78 ± 0.63)% and(16.05 ± 1.02)%, respectively. These effects were associated with an increased expression of cleaved-Caspase-3 and altered expression of bcl-2, bax genes. Thus, icotinib may plays a role in inhibiting proliferation, and increasing apoptosis of schwannoma cells.
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