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Position: Home > Articles > Biological Characters and Molecular Phylogenesis of Five Microsporidian Isolates from Field Insects Science of Sericulture 2012,38 (5) 99-107

从野外昆虫体内分离的5株微孢子虫的生物学特性与分子系统发育研究

作者：

黄旭华;祁广军;汤庆坤;潘志新;朱方容

单位：

广西壮族自治区蚕业技术推广总站;广西蚕业技术推广总站;广西区蚕业技术推广总站

关键词：

野外昆虫;微孢子虫;生物学特性;分子系统发育

摘要：

从广西蚕区的野外昆虫菜粉蝶(Pieris rapae)、桑尺蠖(Hemerophila atrilineata)、斜纹夜蛾(Spodoptera litura)的体内收集到5株对家蚕有一定食下感染力的微孢子虫,通过生物学性状、分子系统发育研究,为其分类鉴定提供依据。来自野外昆虫的5株微孢子虫均具有Nosema属的生物学特性:显微镜下观察形状均呈长卵圆形,与家蚕微孢子虫(Nosema bombycis,Nb)有较明显差异;在生活史的各发育阶段均为双核,以二分裂方式增殖,最后形成双核成熟孢子;与Nb抗血清均产生阳性凝聚反应;超微结构观察均具双核,极丝圈数在12～14圈之间,极丝倾斜角与Nb有差异。根据5株野外昆虫微孢子虫与其它昆虫微孢子虫基于SSU rRNA基因序列构建的系统发育树,以及基于SSU rRNA和rRNA ITS基因序列进行的相似性和遗传距离分析,初步认为5株野外昆虫微孢子虫与家蚕微孢子虫同属异种。

译名：

Biological Characters and Molecular Phylogenesis of Five Microsporidian Isolates from Field Insects

作者：

HUANG Xu-Hua1 QI Guang-Jun1 TANG Qing-Kun1 PAN Zhi-Xin1 ZHU Fang-Rong1 HUANG Yuan-Jiao2 LIN Qiang1 LUO Mei-Lan1 SHI Mei-Ning1 WU Yong-Hu2 HUANG Shen-Hui1(1The General Extension Station of Sericulture Technology of Guangxi Zhuang Autonomous Region,Nanning 530007,China;2Guangxi Medical University,Nanning 530021,China)

关键词：

Field insect;Microsporidia;Biological character;Molecular phylogenesis

摘要：

In this study,5 microsporidian isolates with certain peroral infectivity to Bombyx mori were collected from three field insect species namely Pieris rapae,Hemerophila atrilineata and Spodoptera litura,in Guangxi sericultural areas.Studies on biological characters and molecular phylogenesis were carried out to provide guidance for their taxonomy.It was found that all 5 microsporidian isolates from field insects had the biological characters of genus Nosema.Their spores were all in long oval shape under microscope,which were obviously different from that of Nosema bombycis(Nb).Their nuclei were present in pair at all developmental phases of life cycle,propagated in binary fission,and then developed into two mature spores.Positive coagulation reaction was observed between all 5 microsporidian iso-lates and Nb antiserum.Ultrastructural observation show-ed that all 5 microsporidian cells were binuclear and had 12~14 polar filament coils.Tilt angles of polar filament were different with Nb.Phylogenetic tree based on SSU rRNA sequences and analyses on similarity and genetic distance between SSU rRNA and rRNA ITS sequences of 5 microsporidian isolates and other insect microsporidian species indicated that these 5 microsporidian isolates and Nb belong to different species of the same genus.

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从野外昆虫体内分离的5株微孢子虫的生物学特性与分子系统发育研究

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