Chen Chang-long;Liu Shu-sen;Liu Qian;Niu Jun-hai;Liu Pei;Zhao Jian-long;Liu Zhi-yong;Li Hong-jie;Jian Heng

China Agr Univ, Dept Plant Genet & Breeding, Beijing 100193, Peoples R China;Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China;Chinese Acad Trop Agr Sci, Trop Crops Genet Resources Inst, Danzhou 571737, Peoples R China;Minist Agr, Hebei Acad Agr & Forestry Sci, IPM Ctr Hebei Prov, Key Lab IPM Crops Northern Reg North China,Plant, Baoding 07100, Peoples R China;China Agr Univ, Dept Plant Pathol, Beijing 100193, Peoples R China;Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China

susceptibility identification;Brachypodium distachyon;cereal cyst nematode;Heterodera avenae;host

Cereal cyst nematode (Heterodera avenae, CCN) distributes worldwide and has caused severe damage to cereal crops, and a model host will greatly aid in the study of this nematode. In this research, we assessed the sensitivity of 25 inbred lines of Brachypodium distachyon to H. avenae from Beijing, China. All lines of B. distachyon were infested by second-stage juveniles (J2s) of H. avenae from Daxing District of Beijing population, but only 13 inbred lines reproduced 0.2-3 cysts/plant, showing resistance. The entire root system of the infested B. distachyon appeared smaller and the fibrous roots were shorter and less numerous. We found that a dose of 1 000 J2s of H. avenae was sufficient for nematode infestation. We showed that Koz-1 of B. distachyon could reproduce more cysts than TR2A line. Line Koz-1 also supported the complete life cycles of 5 CCN geographical populations belonging to the Ha1 or Ha3 pathotype group. Our results suggest that B. distachyon is a host for CCN.