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当前位置: 首页 > 文章 > 猪冷冻精液体外受精研究 畜牧兽医学报 2008,39 (8) 1045-1049
Position: Home > Articles > Studies on in vitro Fertilization of Porcine Frozen Semen Chinese Journal of Animal and Veterinary Sciences 2008,39 (8) 1045-1049

猪冷冻精液体外受精研究

作  者：
丁义;戴建军;吴华莉;张德福;徐刚毅
单  位：
上海农业科学院兽医研究所;四川农业大学动物科技学院
关键词：
猪冷冻精液;卵母细胞;体外受精;5毫升大管
摘  要：
为简化猪体外受精过程中精液的来源,避免不同公猪精液和不同采精时间对猪体外受精的影响,本研究采用5 mL大管冷冻精液进行体外受精(IVF),研究IVF过程中的各项参数。结果表明:精卵共孵2~8 h对多精入卵率影响不大(P>0.05),2-细胞率随着共孵时间的延长而增加,8 h时达到72.8%,显著高于2 h组(60.5%,P<0.05),桑椹胚和囊胚发育率以6 h组最高,分别达46.1%和3.48%。多精入卵率随精子浓度的增加而增加,且各组之间差异显著(P<0.05),2-细胞率也以108组(81.2%)最高,且显著高于105组(63.7%)(P<0.05),同时107组和106组分别获得了8.11%和3.13%的囊胚发育率。以NCSU-23作为成熟培养液,并部分去除卵丘细胞的IVF卵裂率和囊胚率最高,达77.1%和7.33%。冻精和新鲜精液的IVF,无论是在卵裂率,还是桑椹胚或囊胚率上均无显著差异(P>0.05)。
译  名：
Studies on in vitro Fertilization of Porcine Frozen Semen
作  者：
DING Yi1,2,3,DAI Jian-jun1,2,WU Hua-li1,2,ZHANG De-fu1,2*,XU Gang-yi3(1.Institute of Veterinary Science,Shanghai Academy of Agricultural Sciences,Shanghai 201106,China;2.Shanghai Municipal Key Laboratory of Agri-genetics and Breeding,Shanghai 201106,China;3.College of Animal Science,Sichuan Agricultural University,Ya'an 625014,China)
关键词：
porcine frozen semen;oocytes;in vitro fertilization;5 mL maxi-straws
摘  要：
In order to simplify the source of semen of in vitro fertilization(IVF),and avoid the effects by different boars and different semen collection time,we used frozen semen by 5 mL-straw to do the IVF.The results showed that there were no significant differences in the polyspermy rates among the 2-8 h oocyte-sperm incubation time(P>0.05).The 2-cell rate was improved along with the increasing of oocyte-sperm incubation time,and 8 h group(72.8%) was much higher than the 2 h group(60.5%,P<0.05).The morula and blastocysts rates of the 6 h group were the highest and got 46.1% and 3.48%,respectively.Polyspermy rate was also increased with the increasing of sperm concentration in the IVF(P<0.05).The 2-cell rate of 108 group(81.2%) was the highest,which was much higher than the 105 group.The 107 and 106 group got 8.11% and 3.13% blastocyst rate,respectively.Using NCSU-23 as the in vitro maturation(IVM) medium and matured oocytes partly denuded cumulus cells could get the highest cleavage and blastocyst rates(77.1%,7.33%) among the four groups.The comparation between the frozen semen and fresh semen in the IVF showed that there were no significant differences in the cleavage,morula and blastocyst rates(P>0.05).

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