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当前位置: 首页 > 文章 > 作物初生根不同发育时期通气组织形成的石蜡切片与徒手切片的比较观察 土壤 2013,45 (5) 946-951
Position: Home > Articles > Comparative Observation of Aerenchyma Formation During Different Crop Primary Root Developmental Stage by Paraffin Section and Freehand Section Soils 2013,45 (5) 946-951

作物初生根不同发育时期通气组织形成的石蜡切片与徒手切片的比较观察

作  者：
张新梅;董晓英;沈仁芳
单  位：
土壤与农业可持续发展国家重点实验室(中国科学院南京土壤研究所)
关键词：
石蜡切片;徒手切片;通气组织;低氧
摘  要：
应用石蜡切片方法和徒手切片方法,通过对水稻幼苗根系通气组织发育的观察,比较两种切片的异同。结果显示:石蜡切片和徒手切片都能清楚显示水稻根系通气组织的结构;并由此确定了水稻幼根通气组织形成过程,具体为:4天苗龄的水稻幼苗根系在培养24 h后开始形成通气组织,72 h时通气组织发育成熟。越靠近根基的部位,植株组织发育更成熟,通气组织的发育程度也更高。相比于石蜡切片,徒手切片方法更适用于水稻根系通气组织结构的观察。进一步利用徒手切片方法比较水稻和小麦根系通气组织形成对低氧的反应,发现低氧显著诱导麦根通气组织的形成,而对稻根通气组织发育的促进作用不明显。
译  名：
Comparative Observation of Aerenchyma Formation During Different Crop Primary Root Developmental Stage by Paraffin Section and Freehand Section
作  者：
ZHANG Xin-mei;DONG Xiao-ying;SHEN Ren-fang;State Key Laboratory of Soil and Sustainable Agriculture (Institute of Soil Science,Chinese Academy of Sciences);University of Chinese Academy of Sciences;
关键词：
Paraffin section,Freehand section,Aerenchyma,Hypoxia
摘  要：
We observed the aerenchyma formation in rice primary root and compared the images obtained by paraffin section and freehand section methods. The results showed that, both of the section types were able to display the structure of rice root and the formation of aerenchyma tissue clearly. The aerenchyma in roots of 4-d old rice seedlings began to form after 24 h growth, and came to completely mature by 72 h. The more closer to the root basal portion, the more mature of the tissue,consequently the higher degree of the aerenchema development. Compared to paraffin section method, the freehand section method is more suitable for observation of the architecture of aerenchyma in rice root. Aerenchyma production in rice and wheat root upon hypoxia observed by freehand section method showed that hypoxia can significantly induce aerenchyma formation in wheat roots, whereas the promoting function in rice roots is not significant.

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