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植物病虫害研究(英文版)
2016,7
(1)
Position: Home > Articles > Establishment of a Real-time Fluorescent Quantita- tive RT-PCR Method for Pineapple mealybug wilt as. sociated virus-2
Plant Diseases and Pests
2016,7
(1)
Establishment of a Real-time Fluorescent Quantita- tive RT-PCR Method for Pineapple mealybug wilt as. sociated virus-2
作 者：
Zhun Fan;Hongyan;}$ Hu$^{;Jiayi;Bin Luo;Zhiwen;Youguang Guo;Lijun Lijun;Jian He;Shu;Fuchu;Qing Li;Xianghong;Bo Zhang;Zhi-y
关键词：
pmwav;rt;pineapple mealybug wilt;reproducibility;sample;qpc
摘 要：
Pineapple mealybug wilt associated virus-2 (PMWaV-2) is an important pathogen causing Mealybug wilt of pineapple (MWP). We established a realtime quantitative RT-PCR( RT-qPCR) method with Taq Man probe based on specific primers of conserved nucleotide sequence of PMWaV-2 coat protein gene. The results showed that the method was highly sensitive to positive sample,but had no fluorescence signal to health sample and blank control. The sensitivity of RT-qPCR was about 100 times higher than general PCR. Reproducibility test revealed that the coefficients of variation between intra-and inter-assay were both within 1.85%,indicating it was a quantitative detection method for PMWaV-2 with simple operation,strong specificity,high sensitivity,and reliable reproducibility.
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