当前位置: 首页 > 文章 > 响应面试验优化超声辅助提取连钱草多糖工艺及其体外抗氧化活性 食品科学 2016,37 (4) 13-19
Position: Home > Articles > Optimization by Response Surface Methodology of Ultrasound-Assisted Extraction and Antioxidant Activities of Polysaccharides from Glechoma longituba(Nakai) Kupr. FOOD SCIENCE 2016,37 (4) 13-19

响应面试验优化超声辅助提取连钱草多糖工艺及其体外抗氧化活性

作  者:
刘晓鹏;张俊霞;姜宁;宋宜枝;向极钎;王秋霜;吴皓
单  位:
南京医药股份有限公司博士后工作站;湖北民族学院生物科学与技术学院;南京中医药大学博士后流动站
关键词:
连钱草;多糖;超声辅助提取;响应面分析;抗氧化
摘  要:
通过中心复合试验优化超声辅助提取连钱草多糖的工艺,以1,1-二苯基-2-苦肼基(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基和2,2’-连氮基-双-(3-乙基苯并噻唑-6-磺酸)自由基(2,2’--azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid)radical,ABTS~+·)清除能力、Fe~(2+)螯合力、铁离子还原抗氧化力(ferric reducing antioxidant power,FRAP)和N,N-二甲基-对苯二胺(N,N-dimethyl-p-phenylenediamine,DMPD)自由基清除能力为指标,研究连钱草多糖的体外抗氧化活性。结果表明,超声辅助提取连钱草多糖的最优工艺为pH 7.2、液固比32∶1(mL/g)、超声功率270 W、超声时间8 min,此条件下多糖提取率在4.95%~5.12%范围内。体外抗氧化活性结果显示,连钱草多糖DPPH自由基清除能力为(0.51±0.04)μmol Trolox/mg,ABTS~+·清除能力为(0.69±0.04)μmol Trolox/mg,Fe~(2+)螯合力为(0.51±0.29)μmol EDTA/mg,FRAP值为(3.45±0.03)μmol Trolox/mg,DMPD自由基清除能力为(0.17±0.01)μmol Trolox/mg。
译  名:
Optimization by Response Surface Methodology of Ultrasound-Assisted Extraction and Antioxidant Activities of Polysaccharides from Glechoma longituba(Nakai) Kupr.
作  者:
LIU Xiaopeng;ZHANG Junxia;JIANG Ning;SONG Yizhi;XIANG Jiqian;WANG Qiushuang;WU Hao;College of Biological Science and Technology,Hubei University for Nationalities;Postdoctoral Research Station,Nanjing Medical Co.Ltd.;Postdoctoral Research Station,Nanjing University of Chinese Medicine;
关键词:
Glechoma longituba(Nakai) Kupr;;polysaccharides;;ultrasound-assisted extraction;;response surface methodology;;antioxidant activity
摘  要:
In this study,we optimized the ultrasound-assisted extraction of polysaccharides from the dried aboveground part of Glechoma longituba(Nakai) Kupr. by response surface methodology(RSM) based on central composite design and measured the antioxidant activities of the extracted polysaccharides using 1,1-diphenyl-2-picrylhydrazyl(DPPH) radical scavenging,2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid radical(ABTS~+·) scavenging,Fe~(2+) chelating activity,ferric reducing antioxidant power(FRAP),and N,N-dimethyl-p-phenylenediamine radical scavenging methods. The optimal extraction conditions were obtained as follows: pH,7.2; liquid-to-solid ratio,32:1(mL/g); ultrasonic power,270 W; and ultrasonic time,8 min. Under these conditions,the yield of polysaccharides was 4.95%–5.12%. The extracted polysaccharides exhibited powerful antioxidant activities. The DPPH radicals and ABTS~+· scavenging activities,Fe~(2+) chelating activity,FRAP value and DMPD radical scavenging capacity were(0.51 ± 0.04) μmol Trolox equivalent(TE)/mg,(0.69 ± 0.04) μmol TE/mg,(0.51 ± 0.29) μmol EDTA-2Na equivalent(EE)/mg,(3.45 ± 0.03) μmol TE/mg and(0.17 ± 0.01) μmol TE/mg,respectively.

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