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当前位置: 首页 > 文章 > 液相色谱-串联质谱联用法测定干海参中硝基呋喃代谢物残留量 大连海洋大学学报 2013,28 (6) 104-106
Position: Home > Articles > Determination of antibiotics nitrofuran metabolites in dried sea cucumber by HPLC-MS / MS Journal of Dalian Ocean University 2013,28 (6) 104-106

液相色谱-串联质谱联用法测定干海参中硝基呋喃代谢物残留量

作  者：
郭无瑕;李肖斐
单  位：
大连标准检测技术研究中心
关键词：
高效液相色谱-串联质谱法;干海参;硝基呋喃代谢物
摘  要：
建立了检测干海参中4种硝基呋喃代谢物的高效液相色谱-串联质谱方法。将干海参样品经过盐酸水解,以2-硝基苯甲醛(2-NBA)为衍生剂,经HLB固相萃取净化后,用高效液相色谱-串联质谱(ESI+)MRM检测,同位素内标定量。结果表明:4种硝基呋喃代谢物的线性范围均为0.5~10.0μg/L,检出限均为0.5μg/kg,3个水平的加标回收率为76.4%~88.4%。该方法稳定性强、重现性好,能满足干海参中4种硝基呋喃代谢物残留检测的要求。
译  名：
Determination of antibiotics nitrofuran metabolites in dried sea cucumber by HPLC-MS / MS
作  者：
GUO Wu-xia;LI Xiao-fei;Dalian Center of Standard Detection Technology;
关键词：
HPLC-MS /MS;;dried sea cucumber;;nitrofuran metabolite
摘  要：
A method for detection of 4 metabolites of antibiotics nitrofuran was developed in dried sea cucumber by HPLC-MS / MS. Samples of the dried sea cucumber were hydrolyzed by hydrochloric acid and derived with 2-nitrobenzaldehyde,was purified by HLB solid-phase extraction,and analyzed by HPLC-MS / MS( ESI+) using multiple reaction monitoring( MRM) in which the quantitative analysis was carried out by isotope internal standard dilution technique. The metabolite levels of antibiotics nitrofuran were shown a good linear relationship over the concentration ranging from 0. 5 μg / L to 10. 0 μg / L,with the detection limit of 0. 5 μg / kg,and average recovery from 76. 4% to 88. 4%,indicating that the method has strong stability,good reproducibility,and meet the requirements for detection of nitrofuran metabolites in dried sea cucumber.

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