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Position: Home > Articles > Experiment on the Separation of Rabbits Sperm using Percoll Density Gradient Centrifugation Chinese Journal of Animal Science 1992 (5)

梯度离心分离家兔精子试验

作  者:
唐克礼;张李俊;梁全忠;侯福安;李文岗;任克良
单  位:
山西省农业科学院畜牧兽医研究所
关键词:
梯度离心;兔精子
摘  要:
用Percoll梯度离心分离家兔精子,收取梯度管上、中、下各层精子给母兔人工授精。上层精子得到10窝共62只仔兔,其中雌性29只(46.8%),雄性33只(53.2%),中层精子得到5窝共31只仔兔,其中雌性16只(51.6%),雄性15只(48.4%);下层精子得到12窝共61只仔兔,其中雌性39只(64.0%),雄性22只(36.0%)。
译  名:
Experiment on the Separation of Rabbits Sperm using Percoll Density Gradient Centrifugation
作  者:
Tang Keli (Institute of Animal Science and Veterinary, Shanxi Academy of Agriculture Science 030032)
关键词:
Gradient centrifugation、 Rabbit Sperm
摘  要:
Rabbits sperm was separated using Percoll density gradient (10 degrees) with centrifuge (250g, 30min) . Each fraction of the degree tube was collected and artificial insemination was made. The rate of female newborn rabbits was 46.8% (29/62) with the upper fraction (1, 2, 3 degree) , 51.6% (16/31) with the middle fraction (4, 5 degree) and 64.0% (39/61) with the lower fraction (8, 9, 10 degree) respectively.

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