当前位置: 首页 > 文章 > 高效液相串联质谱法测定动物源食品中喹赛多、卡巴氧残留 福建农林大学学报(自然科学版) 2008 (3) 307-311
Position: Home > Articles > Determination of cyadox,carbadox in animal origin foodstuffs by liquid chromatography-electrospray tandem mass spectrometry Journal of Fujian Agriculture and Forestry University(Natural Science Edition) 2008 (3) 307-311

高效液相串联质谱法测定动物源食品中喹赛多、卡巴氧残留

作  者:
叶松生;吴德峰;刘正才;杨方;李耀平;林永辉;余孔捷;卢声宇
单  位:
福建农林大学动物科学学院;福建出入境检验检疫局
关键词:
液相色谱—串联质谱法;喹赛多;卡巴氧;残留检测;动物源性食品
摘  要:
本试验建立了动物源食品中喹喔啉类药物喹赛多、卡巴氧残留量的液相色谱—电喷雾串联质谱测定方法.采用酸性乙腈—甲醇混合溶剂提取样品中的残留物,提取液浓缩后经HLB固相萃取小柱净化,以液相色谱—串联质谱仪测定,外标法定量.结果表明:喹赛多、卡巴氧质量浓度为1-50μg.L-1时具有良好的线性范围,相关系数>0.999;在5-20μg.kg-13个添加水平范围内的回收率为69.10%-93.54%,相对标准偏差为2.49%-14.45%,方法检出限分别为3和2μg.kg-1.可见,本方法适用于动物源性样品中喹喔啉类药物残留的定量测定.
译  名:
Determination of cyadox,carbadox in animal origin foodstuffs by liquid chromatography-electrospray tandem mass spectrometry
作  者:
YE Song-sheng1,WU De-feng1,LIU Zheng-cai2,YANG Fang2,LI Yao-ping2,LIN Yong-hui2,YU Kong-jie2,LU Sheng-yu2(1.College of Animal Science,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China;2.Fujian Entry-exit Inspection and Quarantine Bureau,Fuzhou,Fujian 350001,China)
关键词:
liquid chromatography-tandem mass spectrometry;cyadox;carbadox;residues determination;animal origin foodstuffs
摘  要:
A reversed-phase high-performace liquid chromatographic method with tandem mass-spectrometric detection was developed for the simutaneous analysis of cyadox and carbadox in animal origin foodstuffs.The analyses were extracted with acidified acetonitrile and methanol,and then cleaned up with HLB solid phase extraction(SPE) cartridges,followed by liquid chromatography-tandem mass spectrometry analysis.The linear ranges of the cyadox,carbadox were 150 μg·L-1 and the linear correlation coefficient r>0.999.In the ranges of 5-20 μg·kg-1,mean fortification recoveries were 69.10%-93.54%,the relative standard deviation were 2.49%-14.45%.Limits of detection were 3 and 2 μg·kg-1.The results showed that the established method was quite suitable for identification and quantization of quinoxalines.

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