译 名：
Tissue Culture and Plantlet Regeneration of Tadescantia fluminensis
作 者：
CHEN Bao-xin,WANG Xiao-xu,ZHANG Qian-yi,WU Jun,JIANG Chang-yang(College of Life Science, Liaoning Normal University, Dalian,Liaoning 116029, China)
关键词：
Tradescantia fluminensis; Tissue culture; Medium
摘 要：
Tissue culture and plantlet rgeneration of Tadescantia fluminensis with the stem as an explant was studied in order to prove the optimum conditions of callus induction, the differentiation of buds, rooting and transplantation. The results showed that the medium 1/2MS+BA 0.5 mg/L+NAA 0.2 mg/L was the best for inducing callus;MS+BA 1.0 mg/L+2,4-D 0.5 mg/L was the most suitable medium for the callus to differentiate; The opmium medium for forming callus and differentiation of adventitious bud was 1/2MS+BA 0.2 mg/L+NAA 0.1 mg/L;1/2MS+IAA 0.3 mg/L was the ideal for rooting. Using cider as seeding matrix, the surviving rate of transplantation could reach 98% and that of graftage was 91% respectively.
