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药用植物研究(英文版)
2010,1
(8)
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Medicinal Plant
2010,1
(8)
Determination of Coixol in Root,Testa and Stem of Coix lacryma-jobi L.var.ma-yuen(Roman.)Stapf by HPLC
作 者：
Zhang Ming-chang;Ma XiuPing;Wen-Fen Xu;Tian Tian;Jia Xianshen
单 位：
Guiyang College of Traditional Chinese Medicine,Guiyang,Guizhou 55002,China;Guiyang College of Traditional Chinese Medicine,Guiyang,Guizhou 55002,Chin
关键词：
coixol;roman;coix lacryma-jobi l.;ma;stapf;va
摘 要：
[Objective]To establish a method for the determination of coixol in root,stem and testa of Coix lacryma-jobi L.var.ma-yuen(Roman.)Stapf herbs by HPLC.[Method]HPLC was used and the conditions were as follows:Agilent HC-C18 column(4.6 mm×250 mm,5 μm),mobile phase of acetonitrile-0.1% phosphoric acid(V:V=25:75),flow rate of 1.0 ml/min,column temperature of 25 ℃ and detection wavelength of 232 nm.[Result] The contents of coixol in different parts of Coix lacryma-jobi L.var.ma-yuen(Roman.)Stapf.were as follows:roottestastem.[Conclusion]This method is accurate and specific,which is useful for the integrative exploitation of coixol in root,testa and stem of Coix lacryma-jobi L.var.ma-yuen(Roman.)Stapf.
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