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当前位置: 首页 > 文章 > Agrobacterium tumefaciens-mediated transformation of modern rose (Rosa hybrida) using leaf-derived embryogenic callus 园艺学报(英文版) 2021,7 (4)
Position: Home > Articles > Agrobacterium tumefaciens-mediated transformation of modern rose (Rosa hybrida) using leaf-derived embryogenic callus Horticultural Plant Journal 2021,7 (4)

Agrobacterium tumefaciens-mediated transformation of modern rose (Rosa hybrida) using leaf-derived embryogenic callus

作  者：
Guoqin Liu;Yuan Yuan;Hui Jiang;Ying Bao;Guogui Ning;Lingxia Zhao;Xiaofeng Zhou;Zhou Hou-gao;Junping Gao;Nan M
单  位：
State Key Laboratory of Agrobiotechnology, Beijing Key Laboratory of Development and Quality Control of Ornamental Crops, Department of Ornamental Horticulture, College of Horticulture, China Agricultural University, Beijing 100193, Chin;State Key Laboratory of Agrobiotechnology, Beijing Key Laboratory of Development and Quality Control of Ornamental Crops, Department of Ornamental Horticulture, College of Horticulture, China Agricultural University, Beijing 100193, China;Faculty of Life Science, Tangshan Normal University, Tangshan, Hebei 063000, China;Key Laboratory of Horticultural Plant Biology, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China;College Horticulture & Landscape Architecture, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China
关键词：
Rose;Rosa hybrida;Leaf;Somatic embryos;Agrobacterium tumefaciens;Genetic transformatio
摘  要：
Rose (Rosa hybrida) is widely used for cut flowers and as garden plants. Stable and efficient transformation system is required for functional genomics of rose. Here, we established an efficient transformation method for rose using Agrobacterium tumefaciens-mediated transformation of embryogenic callus. Expanding rose leaves were used as explants to induce somatic embryos, which were subjected to transformation with A. tumefaciens strain GV3101 using Green Fluorescence Protein (GFP) as a marker gene. It took about 8 months to generate transgenic shoots from embryogenic callus. PCR, RT-PCR, Southern and Western blotting, as well as stereoscopic fluorescence microscopy analysis demonstrated that GFP transgenes integrated stably into the rose genome. According to our data, a transformation efficiency of up to 6% can be achieved by following this optimized protocol.

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