当前位置: 首页 > 文章 > 阴沟肠杆菌(Enterobacter closcae)对乐果降解特性的研究 农业环境科学学报 2009,28 (02) 184-191
Position: Home > Articles > Degradation Characteristics of Dimethoate by Enterobacter closcae Journal of Agro-Environment Science 2009,28 (02) 184-191

阴沟肠杆菌(Enterobacter closcae)对乐果降解特性的研究

作  者:
林抗美;官雪芳;鲁国东;王宗华;张秋芳;谢恩;马丽娜;刘波
单  位:
福建农林大学生命科学学院;福建省农科院农业生物资源研究所;福建省农科院农业工程技术研究所
关键词:
阴沟肠杆菌;乐果;降解;优化
摘  要:
从福建省福农生化有限公司排污口的土壤中筛选到一株对乐果有较好降解效果的菌株阴沟肠杆菌,为了使该菌能更好的应用于乐果残留污染的治理当中,采用室内培养方法,对该菌降解乐果的作用因素进行研究。结果表明,菌降解乐果的最佳温度为35℃、pH为8.0、装液量50mL.250mL-1、摇床振数180r.min-1、C源为果糖、N源为酵母浸膏、接种量OD265为0.065、底物浓度为200mg.L-1,对应的降解率在28.84%~61.19%之间,采用L1(82×37)正交法及DPS数理统计得出Cu2+、Fe2+对菌降解乐果有显著的促进效果,Cd2+、Zn2+则在溶液中含量低水平时促进降解,高水平时抑制降解;Mg2+、Ba2+、Mn2+、Pb2+等离子对菌降解乐果无促进作用。
译  名:
Degradation Characteristics of Dimethoate by Enterobacter closcae
作  者:
LIN Kang-mei1,GUAN Xue-fang2,LU Guo-dong3,WANG Zong-hua3,ZHANG Qiu-fang1,XIE En3,MA Li-na1,LIU Bo1(1.Agricultural Biological Resources Research Institute,Fujian Academy of Agricultural Sciences Fuzhou 350003,China;2.Institute of A-gricultural Engineering Technology,Fujian Academy of Agricultural Sciences,Fuzhou 350003,China;3.Life Science College,Fujian Agri-culture and Forestry University,Fuzhou 350002,China)
关键词:
Enterobacter closcae;dimethoate;degradation;optimize
摘  要:
By using selective cultural media,Enterobacter closcae was isolated to degrade dimethoate from the soil around the pesticide fac-tory.The factors affecting the degradation of dimethoate by E.closcae were studied.The results showed that the optimum temperature for the degradation was 35 ℃ for the growth of the strain,the initial pH was 8.0,the conformable carbon source was fructose,the conformable nitro-gen source was the yeast extract,the inoculum's size was OD265:0.065,the density of dimethoate was 200 mg.L-1,the air supply was 50 mL.250 mL-1 volume in the cultural bottle with 180 r.min-1 speed in the rotated shaker,in which the degrading rates to dimethoate were ranged in 28.84%~61.19%.The L1(82×37)orthogonal test showed that the ion Cu2+ and Fe2+ could promote the strain to degrade dimethoate,and Cd2+、 Zn2+ could also promote it when it was at low level.However,Mg2+,Ba2+,Mn2+,Pb2+ had no effect on the degradation of dimethoate.

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