Screening and Construction of SU-DHL Aptamers for Human B-cell Lymphoma Based on Cell-SELEX Technology

YU Wenying;WANG Mengzhu;DU Bingyang;WANG Chang;GAO Lijun;XIA Wei;College of Medical Technology,Beihua University;Xiantao First People's Hospital,Yangtze University;Jilin Central Hospital;

SU-DHL-8 cells;;lymphoma;;aptamer;;SELEX technology

Objective To screen the aptamers that specifically bind to lymphoma SU-DHL-8 cells for early diagnosis of lymphoma.Method 76 bp random single-stranded DNA library was used, with SU-DHL-8 cells as the target cells and Ramos cells as the subtractive cells.The ligand system evolution technology with exponential enrichment was used to screen and enrich the aptamers that bind to the target cells; The secondary library was prepared by PCR and biotin-streptavidin chromatography.After multiple rounds of screening, the aptamer speci-fically binding to SU-DHL-8 cells was finally selected.Results After 11 rounds of forward screening and 2 rounds of reverse screening, the affinity between the secondary ssDNA library and SU-DHL-8 cells gradually increased, and the fluorescence curves of the 12th and 13th rounds basically coincided, indicating that the specific aptamer of SU-DHL-8 cells was successfully constructed.Conclusion The Cell-SELEX technique can successfully screen the specific aptamer of lymphoma SU-DHL-8 cells, and the new diagnostic marker of lymphoma can be obtained by labeling the probe, which provides the basis for accurate and early diagnosis of lymphoma.