Position: Home > Articles > Preservation Conditions of Vibrio parahaemolyticus
Hubei Agricultural Sciences
2014,53
(18)
4395-4398
副溶血性弧菌保藏条件研究
作 者：
杨胜远;韦锦;李卓文;方晓旋;赖演莲;杨娜;陈晓屏
单 位：
韩山师范学院图书馆;韩山师范学院生物学系
关键词：
副溶血性弧菌(Vibrio parahaemolyticus);甘油;培养基;NaCl
摘 要：
针对副溶血性弧菌(Vibrio parahaemolyticus)在低温条件下容易进入不可培养状态,难以保藏的问题,通过平板菌落计数法对副溶血性弧菌的4℃冰箱保藏条件进行了探讨。结果表明,NaCl对副溶血性弧菌4℃保藏有保护作用,其中以45 g/L NaCl保护效果最佳,而且45 g/L NaCl不影响副溶血性弧菌的生长。将副溶血性弧菌细胞浸于45 g/L NaCl溶液或以含45 g/L NaCl的培养基培养后直接于4℃保藏,保藏90 d,活细胞数仅由109下降到106。
译 名：
Preservation Conditions of Vibrio parahaemolyticus
作 者：
YANG Sheng-yuan;WEI Jin;LI Zhuo-wen;FANG Xiao-xuan;LAI Yan-lian;YANG Na;CHEN Xiao-ping;Department of Biology Hanshan Normal University;library Hanshan Normal University;
关键词：
Vibrio parahaemolyticus;;glycerin;;medium;;NaCl
摘 要：
Considering that Vibrio parahaemolyticus is easy to be viable but nonculturable at low temperature, the preservation conditions of V. parahaemolyticus at 4 ℃ were investigated with plate count method. The results showed that NaCl was very helpful for V. parahaemolyticus preservation at 4 ℃. The optimal concentration of NaCl for the V.parahaemolyticus protection was 45 g / L. 45 g / L of NaCl had no negative effects on the growth of V. parahaemolyticus. When preserved V. parahaemolyticus at 4 ℃ by immersed the cells in 45 g / L of NaCl or directly used the liquid cultures cultivated with the liquid media containing 45 g / L of NaCl, the number of culturable cells were slightly decreased from initial 109 to 106after 90 d.
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